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Assay Detail

CHEMBL4385276

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant human HL expressed in COS7 cells using PED-A1 containing DMPG vesicles as substrate pretreated for 20 mins followed by substrate addition and measured every 20 secs for 10 mins by fluorescence assay
16
Total Activities
15
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Hepatic triacylglycerol lipase (CHEMBL2127)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Sulfonylated Benzothiazoles as Inhibitors of Endothelial Lipase.
Johnson JA, Tora G, Pi Z, Phillips M, Yin X, Yang R, Zhao L, Chen AY, Taylor DS, Basso M, Rose A, Behnia K, Onorato J, Chen XQ, Abell LM, Lu H, Locke G, Caporuscio C, Galella M, Adam LP, Gordon D, Wexler RR, Finlay HJ.
ACS Med Chem Lett (2018) 9 : 1263 -1268
PubMed 30613337 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 16 7.01 8.52

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL175247 ORLISTAT 4.0 1 8.52
CHEMBL4440746 1 8.00
CHEMBL4468090 1 7.68
CHEMBL4536805 1 7.28
CHEMBL4471545 1 7.28
CHEMBL4473964 1 7.05
CHEMBL4435864 1 6.92
CHEMBL4577903 1 6.41
CHEMBL4563513 1 6.35
CHEMBL4516251 2 5.85
CHEMBL4546575 1 -
CHEMBL4567596 1 -
CHEMBL4473238 1 -
CHEMBL4589925 1 -
CHEMBL4458141 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL175247 ORLISTAT IC50 = 3.0 nM 8.52
CHEMBL4440746 IC50 = 10.0 nM 8.00
CHEMBL4468090 IC50 = 21.0 nM 7.68
CHEMBL4536805 IC50 = 53.0 nM 7.28
CHEMBL4471545 IC50 = 53.0 nM 7.28
CHEMBL4473964 IC50 = 90.0 nM 7.05
CHEMBL4435864 IC50 = 121.0 nM 6.92
CHEMBL4577903 IC50 = 391.0 nM 6.41
CHEMBL4563513 IC50 = 450.0 nM 6.35
CHEMBL4516251 IC50 = 1400.0 nM 5.85
CHEMBL4516251 IC50 = 1900.0 nM 5.72
CHEMBL4546575 IC50 < 10.0 nM -
CHEMBL4567596 IC50 < 10.0 nM -
CHEMBL4473238 IC50 < 10.0 nM -
CHEMBL4589925 IC50 < 10.0 nM -
CHEMBL4458141 IC50 < 10.0 nM -