Assay Detail
Binding
CHEMBL4385495
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of BTK in goat anti-human IgM F(ab')2-stimulated human whole blood assessed as suppression of CD69 expression on B cells pretreated for 1 hr followed by goat anti-human IgM F(ab')2-stimualtion and measured after 3 hrs by Fix/Lyse reagent based flow cytometry
5
Total Activities
5
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Tissue | Blood |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Aminopyrazole Carboxamide Bruton's Tyrosine Kinase Inhibitors. Irreversible to Reversible Covalent Reactive Group Tuning.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 5 | 7.30 | 7.48 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL4456974 | — | — | 1 | 7.48 |
| CHEMBL4469663 | — | — | 1 | 7.35 |
| CHEMBL4456283 | — | — | 1 | 7.34 |
| CHEMBL4560385 | — | — | 1 | 7.28 |
| CHEMBL4462332 | — | — | 1 | 7.05 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL4456974 | — | IC50 | = | 33.0 | nM | 7.48 |
| CHEMBL4469663 | — | IC50 | = | 45.0 | nM | 7.35 |
| CHEMBL4456283 | — | IC50 | = | 46.0 | nM | 7.34 |
| CHEMBL4560385 | — | IC50 | = | 53.0 | nM | 7.28 |
| CHEMBL4462332 | — | IC50 | = | 89.0 | nM | 7.05 |