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Assay Detail

CHEMBL4386801

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Cis-inhibition of human LAT1 expressed in TREx HEK293 cells assessed as inhibition of [3H]-gabapentin uptake preincubated for 3 mins at 37 degC followed by washing with choline buffer and measured after 3 hrs by scintillation counting analysis
16
Total Activities
16
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Large neutral amino acids transporter small subunit 1 (CHEMBL4459)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

l-Type amino acid transporter 1 activity of 1,2,3-triazolyl analogs of l-histidine and l-tryptophan.
Hall C, Wolfe H, Wells A, Chien HC, Colas C, Schlessinger A, Giacomini KM, Thomas AA.
Bioorg Med Chem Lett (2019) 29 : 2254 -2258
PubMed 31248771 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 16 4.11 4.16

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL301523 PHENYLALANINE 3.0 1 4.16
CHEMBL4242404 1 4.16
CHEMBL4249170 1 4.00
CHEMBL4464816 1 -
CHEMBL4448346 1 -
CHEMBL4569744 1 -
CHEMBL4586342 1 -
CHEMBL4587818 1 -
CHEMBL4457506 1 -
CHEMBL4570233 1 -
CHEMBL17962 HISTIDINE 2.0 1 -
CHEMBL1233327 1 -
CHEMBL4240267 1 -
CHEMBL4243343 1 -
CHEMBL54976 TRYPTOPHAN 4.0 1 -
CHEMBL4559029 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL301523 PHENYLALANINE IC50 = 69000.0 nM 4.16
CHEMBL4242404 IC50 = 69000.0 nM 4.16
CHEMBL4249170 IC50 = 100000.0 nM 4.00
CHEMBL4464816 IC50 = 160000.0 nM -
CHEMBL4448346 IC50 > 1000000.0 nM -
CHEMBL4569744 IC50 > 1000000.0 nM -
CHEMBL4586342 IC50 = 440000.0 nM -
CHEMBL4587818 IC50 > 1000000.0 nM -
CHEMBL4457506 IC50 - -
CHEMBL4570233 IC50 - -
CHEMBL17962 HISTIDINE IC50 = 110000.0 nM -
CHEMBL1233327 IC50 = 190000.0 nM -
CHEMBL4240267 IC50 = 170000.0 nM -
CHEMBL4243343 IC50 = 160000.0 nM -
CHEMBL54976 TRYPTOPHAN IC50 = 160000.0 nM -
CHEMBL4559029 IC50 = 340000.0 nM -