Assay Detail
Functional
CHEMBL4387152
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Antiproliferative activity against human BT549 cells assessed as reduction in DNA replication incubated for 16 hours followed by addition of EdU and incubated for 8 hours by Alexa flour488 and HCS dye-based fluorescence imaging method
7
Total Activities
7
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Functional |
| Organism | Homo sapiens |
| Cell Type | BT-549 |
| Confidence | 1 — Organism |
| Curated By | Autocuration |
Publication
Design of a brain-penetrant CDK4/6 inhibitor for glioblastoma.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| EC50 | 7 | 5.41 | 5.70 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL4440980 | — | — | 1 | 5.70 |
| CHEMBL4545456 | — | — | 1 | 5.68 |
| CHEMBL4520067 | — | — | 1 | 5.58 |
| CHEMBL4539133 | — | — | 1 | 5.46 |
| CHEMBL4589135 | — | — | 1 | 5.29 |
| CHEMBL4546255 | — | — | 1 | 4.77 |
| CHEMBL4466797 | — | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL4440980 | — | EC50 | = | 2000.0 | nM | 5.70 |
| CHEMBL4545456 | — | EC50 | = | 2100.0 | nM | 5.68 |
| CHEMBL4520067 | — | EC50 | = | 2600.0 | nM | 5.58 |
| CHEMBL4539133 | — | EC50 | = | 3500.0 | nM | 5.46 |
| CHEMBL4589135 | — | EC50 | = | 5100.0 | nM | 5.29 |
| CHEMBL4546255 | — | EC50 | = | 17000.0 | nM | 4.77 |
| CHEMBL4466797 | — | EC50 | > | 2200.0 | nM | - |