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Assay Detail

CHEMBL4387399

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant Leishmania amazonensis arginase expressed in Escherichia coli assessed as reduction in urea production using L-arginine as substrate by Berthelot colorimetric method relative to control
10
Total Activities
10
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Leishmania amazonensis
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Arginase (CHEMBL3108635)
Type SINGLE PROTEIN
Organism Leishmania amazonensis

Publication

Phenylhydrazides as inhibitors of Leishmania amazonensis arginase and antileishmanial activity.
Crizanto de Lima E, Castelo-Branco FS, Maquiaveli CC, Farias AB, Rennó MN, Boechat N, Silva ER.
Bioorg Med Chem (2019) 27 : 3853 -3859
PubMed 31311700 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 10 4.67 4.92

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4204512 1 4.92
CHEMBL4216180 1 4.92
CHEMBL4530523 1 4.43
CHEMBL4574177 1 4.42
CHEMBL267345 AMPHOTERICIN B 4.0 1 -
CHEMBL4459163 1 -
CHEMBL4546693 1 -
CHEMBL4562829 1 -
CHEMBL4216693 1 -
CHEMBL4211729 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4204512 IC50 = 12000.0 nM 4.92
CHEMBL4216180 IC50 = 12000.0 nM 4.92
CHEMBL4530523 IC50 = 37000.0 nM 4.43
CHEMBL4574177 IC50 = 38000.0 nM 4.42
CHEMBL267345 AMPHOTERICIN B IC50 - -
CHEMBL4459163 IC50 - -
CHEMBL4546693 IC50 - -
CHEMBL4562829 IC50 - -
CHEMBL4216693 IC50 > 100000.0 nM -
CHEMBL4211729 IC50 > 100000.0 nM -