Assay Detail
Binding
CHEMBL4388516
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Inhibition of WEE1 in human NCI-H1299 cells assessed as reduction in Cdc2 phosphorylation at Tyr15 residue measured after 6 hrs by ELISA
15
Total Activities
15
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Cell Type | NCI-H1299 |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Investigation of biaryl heterocycles as inhibitors of Wee1 kinase.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| EC50 | 15 | 7.16 | 7.96 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL4441166 | — | — | 1 | 7.96 |
| CHEMBL4447769 | — | — | 1 | 7.75 |
| CHEMBL4554796 | — | — | 1 | 7.72 |
| CHEMBL4444364 | — | — | 1 | 7.68 |
| CHEMBL4551299 | — | — | 1 | 7.66 |
| CHEMBL4529353 | — | — | 1 | 7.60 |
| CHEMBL4526423 | — | — | 1 | 7.55 |
| CHEMBL4470852 | — | — | 1 | 7.54 |
| CHEMBL4454675 | — | — | 1 | 7.51 |
| CHEMBL3355539 | — | — | 1 | 7.22 |
| CHEMBL4443172 | — | — | 1 | 7.10 |
| CHEMBL4544916 | — | — | 1 | 6.61 |
| CHEMBL4469678 | — | — | 1 | 6.49 |
| CHEMBL4450036 | — | — | 1 | 5.54 |
| CHEMBL4562919 | — | — | 1 | 5.40 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL4441166 | — | EC50 | = | 11.0 | nM | 7.96 |
| CHEMBL4447769 | — | EC50 | = | 18.0 | nM | 7.75 |
| CHEMBL4554796 | — | EC50 | = | 19.0 | nM | 7.72 |
| CHEMBL4444364 | — | EC50 | = | 21.0 | nM | 7.68 |
| CHEMBL4551299 | — | EC50 | = | 22.0 | nM | 7.66 |
| CHEMBL4529353 | — | EC50 | = | 25.0 | nM | 7.60 |
| CHEMBL4526423 | — | EC50 | = | 28.0 | nM | 7.55 |
| CHEMBL4470852 | — | EC50 | = | 29.0 | nM | 7.54 |
| CHEMBL4454675 | — | EC50 | = | 31.0 | nM | 7.51 |
| CHEMBL3355539 | — | EC50 | = | 60.0 | nM | 7.22 |
| CHEMBL4443172 | — | EC50 | = | 79.0 | nM | 7.10 |
| CHEMBL4544916 | — | EC50 | = | 246.0 | nM | 6.61 |
| CHEMBL4469678 | — | EC50 | = | 324.0 | nM | 6.49 |
| CHEMBL4450036 | — | EC50 | = | 2850.0 | nM | 5.54 |
| CHEMBL4562919 | — | EC50 | = | 4020.0 | nM | 5.40 |