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Assay Detail

CHEMBL4388527

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of probe binding to EGFR L858R mutant (unknown origin) using rabbit reticulate lysate system after 1 hr by luminescence assay
25
Total Activities
25
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Epidermal growth factor receptor (CHEMBL203)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Lead generation of 1,2-dithiolanes as exon 19 and exon 21 mutant EGFR tyrosine kinase inhibitors.
Mansour TS, Potluri V, Pallepati RR, Basetti V, Keesara M, Moghudula AG, Maiti P.
Bioorg Med Chem Lett (2019) 29 : 1435 -1439
PubMed 31023512 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 25 7.53 8.85

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL180022 NERATINIB 4.0 1 8.85
CHEMBL554 LAPATINIB 4.0 1 8.74
CHEMBL1173655 AFATINIB 4.0 1 8.70
CHEMBL31965 CANERTINIB 3.0 1 8.57
CHEMBL4582933 1 8.24
CHEMBL4457885 1 8.19
CHEMBL4517633 1 8.05
CHEMBL4457447 1 7.96
CHEMBL4456266 1 7.75
CHEMBL4519305 1 7.70
CHEMBL4531250 1 7.57
CHEMBL4547475 1 7.48
CHEMBL4446124 1 7.44
CHEMBL3353410 OSIMERTINIB 4.0 1 7.42
CHEMBL4519470 1 7.32
CHEMBL553 ERLOTINIB 4.0 1 7.10
CHEMBL939 GEFITINIB 4.0 1 7.08
CHEMBL4441244 1 6.70
CHEMBL4576802 1 6.66
CHEMBL4451538 1 6.52
CHEMBL4463005 1 6.38
CHEMBL4593016 1 6.38
CHEMBL4546553 1 6.31
CHEMBL4551202 1 -
CHEMBL4560998 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL180022 NERATINIB IC50 = 1.4 nM 8.85
CHEMBL554 LAPATINIB IC50 = 1.8 nM 8.74
CHEMBL1173655 AFATINIB IC50 = 2.0 nM 8.70
CHEMBL31965 CANERTINIB IC50 = 2.7 nM 8.57
CHEMBL4582933 IC50 = 5.8 nM 8.24
CHEMBL4457885 IC50 = 6.5 nM 8.19
CHEMBL4517633 IC50 = 9.0 nM 8.05
CHEMBL4457447 IC50 = 10.9 nM 7.96
CHEMBL4456266 IC50 = 18.0 nM 7.75
CHEMBL4519305 IC50 = 20.0 nM 7.70
CHEMBL4531250 IC50 = 26.8 nM 7.57
CHEMBL4547475 IC50 = 33.0 nM 7.48
CHEMBL4446124 IC50 = 36.0 nM 7.44
CHEMBL3353410 OSIMERTINIB IC50 = 38.0 nM 7.42
CHEMBL4519470 IC50 = 48.0 nM 7.32
CHEMBL553 ERLOTINIB IC50 = 80.0 nM 7.10
CHEMBL939 GEFITINIB IC50 = 83.0 nM 7.08
CHEMBL4441244 IC50 = 199.0 nM 6.70
CHEMBL4576802 IC50 = 219.0 nM 6.66
CHEMBL4451538 IC50 = 300.0 nM 6.52
CHEMBL4463005 IC50 = 420.0 nM 6.38
CHEMBL4593016 IC50 = 413.0 nM 6.38
CHEMBL4546553 IC50 = 494.0 nM 6.31
CHEMBL4551202 IC50 > 10000.0 nM -
CHEMBL4560998 IC50 > 10000.0 nM -