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Assay Detail

CHEMBL4392555

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of PRMT5 (unknown origin)/MEP50 (unknown origin) using histone H2 as substrate preincubated for 15 to 20 mins followed by S-[methyl-3H]adenosyl-L-methionine addition measured after 60 mins by liquid scintillation counting
5
Total Activities
5
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 7 — Homologous single protein target
Curated By Autocuration

Target

PRMT5/MEP50 complex (CHEMBL3137261)
Type PROTEIN COMPLEX
Organism Homo sapiens

Publication

Nucleoside protein arginine methyltransferase 5 (PRMT5) inhibitors.
Lin H, Luengo JI.
Bioorg Med Chem Lett (2019) 29 : 1264 -1269
PubMed 30956011 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 5 8.04 9.22

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4536042 1 9.22
CHEMBL4457371 1 8.70
CHEMBL4465375 1 7.77
CHEMBL4554624 1 7.58
CHEMBL4459256 1 6.94

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4536042 IC50 = 0.6 nM 9.22
CHEMBL4457371 IC50 = 2.0 nM 8.70
CHEMBL4465375 IC50 = 17.0 nM 7.77
CHEMBL4554624 IC50 = 26.0 nM 7.58
CHEMBL4459256 IC50 = 116.0 nM 6.94