Assay Detail
Binding
CHEMBL4395300
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of recombinant Escherichia coli BL21(DE3) isoleucyl-tRNA synthetase expressed in Escherichia coli Rosetta 2 (DE3) assessed as reduction in tRNA aminoacylation preincubated for 10 mins in presence of [14C]-isoleucin and tRNA followed by ATP addition and measured after 6 mins by scintillation counting method
2
Total Activities
1
Compounds Tested
2
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Escherichia coli BL21(DE3) |
| Confidence | 8 — Homologous single protein target |
| Curated By | Autocuration |
Publication
Acylated sulfonamide adenosines as potent inhibitors of the adenylate-forming enzyme superfamily.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| Ki | 1 | 8.92 | 8.92 |
| IC50 | 1 | 8.17 | 8.17 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL4553017 | — | — | 2 | 8.92 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL4553017 | — | Ki | = | 1.2 | nM | 8.92 |
| CHEMBL4553017 | — | IC50 | = | 6.8 | nM | 8.17 |