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Assay Detail

CHEMBL4397198

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Binding
Inhibition of recombinant human His6-tagged PDE4D7 UCR1 S129D mutant expressed in baculovirus infected Sf9 insect cells using cAMP as substrate preincubated for 5 to 10 mins followed by substrate addition and measured for 10 mins by yeast myokinase/pyruvate kinase/lactate dehydrogenase-coupled fluorescence assay
27
Total Activities
27
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type Sf9
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

Design and Synthesis of Selective Phosphodiesterase 4D (PDE4D) Allosteric Inhibitors for the Treatment of Fragile X Syndrome and Other Brain Disorders.
Gurney ME, Nugent RA, Mo X, Sindac JA, Hagen TJ, Fox D, O'Donnell JM, Zhang C, Xu Y, Zhang HT, Groppi VE, Bailie M, White RE, Romero DL, Vellekoop AS, Walker JR, Surman MD, Zhu L, Campbell RF.
J Med Chem (2019) 62 : 4884 -4901
PubMed 31013090 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 27 7.74 9.70

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4476440 1 9.70
CHEMBL4570605 1 9.30
CHEMBL4593524 1 9.10
CHEMBL4437670 1 8.96
CHEMBL4573171 1 8.77
CHEMBL4449185 1 8.74
CHEMBL4458210 1 8.70
CHEMBL4459298 1 8.59
CHEMBL4544243 1 8.30
CHEMBL4541964 ZATOLMILAST 3.0 1 8.11
CHEMBL4567525 1 8.07
CHEMBL4440664 1 8.05
CHEMBL4434783 1 7.92
CHEMBL4564798 1 7.66
CHEMBL514800 APREMILAST 4.0 1 7.58
CHEMBL4569780 1 7.46
CHEMBL4472119 1 7.46
CHEMBL4553222 1 7.38
CHEMBL4518460 1 7.29
CHEMBL3315275 1 7.02
CHEMBL4561428 1 6.88
CHEMBL3315270 1 6.67
CHEMBL4455430 1 6.65
CHEMBL4549467 1 6.32
CHEMBL3315269 1 6.17
CHEMBL4533457 1 6.13
CHEMBL4520584 1 5.93

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4476440 IC50 = 0.2 nM 9.70
CHEMBL4570605 IC50 = 0.5 nM 9.30
CHEMBL4593524 IC50 = 0.8 nM 9.10
CHEMBL4437670 IC50 = 1.1 nM 8.96
CHEMBL4573171 IC50 = 1.7 nM 8.77
CHEMBL4449185 IC50 = 1.8 nM 8.74
CHEMBL4458210 IC50 = 2.0 nM 8.70
CHEMBL4459298 IC50 = 2.6 nM 8.59
CHEMBL4544243 IC50 = 5.0 nM 8.30
CHEMBL4541964 ZATOLMILAST IC50 = 7.8 nM 8.11
CHEMBL4567525 IC50 = 8.6 nM 8.07
CHEMBL4440664 IC50 = 9.0 nM 8.05
CHEMBL4434783 IC50 = 12.0 nM 7.92
CHEMBL4564798 IC50 = 22.0 nM 7.66
CHEMBL514800 APREMILAST IC50 = 26.0 nM 7.58
CHEMBL4569780 IC50 = 35.0 nM 7.46
CHEMBL4472119 IC50 = 35.0 nM 7.46
CHEMBL4553222 IC50 = 42.1 nM 7.38
CHEMBL4518460 IC50 = 51.0 nM 7.29
CHEMBL3315275 IC50 = 96.0 nM 7.02
CHEMBL4561428 IC50 = 133.0 nM 6.88
CHEMBL3315270 IC50 = 213.0 nM 6.67
CHEMBL4455430 IC50 = 226.0 nM 6.65
CHEMBL4549467 IC50 = 475.0 nM 6.32
CHEMBL3315269 IC50 = 681.0 nM 6.17
CHEMBL4533457 IC50 = 733.0 nM 6.13
CHEMBL4520584 IC50 = 1165.0 nM 5.93