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Assay Detail

CHEMBL4397304

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of Lysosomal alpha-mannosidase in wild-type human fibroblast cell lysates using 4-methylumbelliferone alpha-D-mannopyranoside as a reporter substrate incubated for 60 mins by fluorescence method
7
Total Activities
7
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Subcellular Lysate
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Lysosomal alpha-mannosidase (CHEMBL4059)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Pharmacological Chaperones for the Treatment of α-Mannosidosis.
Rísquez-Cuadro R, Matsumoto R, Ortega-Caballero F, Nanba E, Higaki K, García Fernández JM, Ortiz Mellet C.
J Med Chem (2019) 62 : 5832 -5843
PubMed 31017416 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 7 5.10 6.36

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4465842 1 6.36
CHEMBL4462117 1 5.66
CHEMBL4475419 1 4.76
CHEMBL4457217 1 4.71
CHEMBL4471561 1 4.57
CHEMBL4518572 1 4.51
CHEMBL4435118 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4465842 IC50 = 440.0 nM 6.36
CHEMBL4462117 IC50 = 2200.0 nM 5.66
CHEMBL4475419 IC50 = 17300.0 nM 4.76
CHEMBL4457217 IC50 = 19300.0 nM 4.71
CHEMBL4471561 IC50 = 26800.0 nM 4.57
CHEMBL4518572 IC50 = 30700.0 nM 4.51
CHEMBL4435118 IC50 = 116000.0 nM -