Assay Detail
Binding
CHEMBL4397829
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Binding affinity to mouse N-terminal His6-Trx-tagged RANKL ectodomain (157 to 316 residues) expressed in Escherichia coli BL21 (DE3) by isothermal titration calorimetric assay
2
Total Activities
2
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Mus musculus |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Tumor necrosis factor ligand superfamily member 11 (CHEMBL3596084) ↗| Type | SINGLE PROTEIN |
| Organism | Mus musculus |
Publication
Development of Small-Molecules Targeting Receptor Activator of Nuclear Factor-κB Ligand (RANKL)-Receptor Activator of Nuclear Factor-κB (RANK) Protein-Protein Interaction by Structure-Based Virtual Screening and Hit Optimization.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| Kd | 2 | 4.76 | 4.76 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL4462272 | — | — | 1 | 4.76 |
| CHEMBL4469489 | — | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL4462272 | — | Kd | = | 17300.0 | nM | 4.76 |
| CHEMBL4469489 | — | Kd | = | 284400.0 | nM | - |