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Assay Detail

CHEMBL4399508

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Noncompetitive inhibition of SMS2 (unknown origin) stably expressed in mouse ZS cells using 5 to 50 uM C6-NBD-ceramide as substrate preincubated for 30 mins followed by substrate addition and measured after 3 hrs by HPLC analysis
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Publication

Malabaricone C as Natural Sphingomyelin Synthase Inhibitor against Diet-Induced Obesity and Its Lipid Metabolism in Mice.
Othman MA, Yuyama K, Murai Y, Igarashi Y, Mikami D, Sivasothy Y, Awang K, Monde K.
ACS Med Chem Lett (2019) 10 : 1154 -1158
PubMed 31413799 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 3 6.00 6.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1254269 MALABARICONE A 1 6.00
CHEMBL489354 MALABARICONE B 1 6.00
CHEMBL524100 MALABARICONE C 1 6.00

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1254269 MALABARICONE A IC50 = 1000.0 nM 6.00
CHEMBL489354 MALABARICONE B IC50 = 1000.0 nM 6.00
CHEMBL524100 MALABARICONE C IC50 = 1000.0 nM 6.00