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Assay Detail

CHEMBL4402292

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of Wistar rat lens ALR2 using L-glyceraldehyde as substrate preincubated for 10 mins followed by substrate addition and measured for 4 mins by spectrophotometric method
22
Total Activities
22
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Aldo-keto reductase family 1 member B1 (CHEMBL2622)
Type SINGLE PROTEIN
Organism Rattus norvegicus

Publication

Designing of acyl sulphonamide based quinoxalinones as multifunctional aldose reductase inhibitors.
Ji Y, Chen X, Chen H, Zhang X, Fan Z, Xie L, Ma B, Zhu C.
Bioorg Med Chem (2019) 27 : 1658 -1669
PubMed 30858026 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 22 5.10 7.51

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL56337 EPALRESTAT 4.0 1 7.51
CHEMBL4445514 1 7.00
CHEMBL3409366 1 6.74
CHEMBL3409369 1 6.38
CHEMBL4476269 1 6.20
CHEMBL4547451 1 5.49
CHEMBL4447350 1 5.33
CHEMBL4553065 1 5.20
CHEMBL4465733 1 4.99
CHEMBL4467988 1 4.85
CHEMBL4570469 1 4.80
CHEMBL4580855 1 4.63
CHEMBL4453241 1 4.47
CHEMBL4592035 1 4.45
CHEMBL4550229 1 4.42
CHEMBL4518301 1 4.41
CHEMBL4471025 1 4.39
CHEMBL4436476 1 4.27
CHEMBL4545610 1 4.26
CHEMBL4564163 1 4.14
CHEMBL4578039 1 4.13
CHEMBL4463496 1 4.12

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL56337 EPALRESTAT IC50 = 31.0 nM 7.51
CHEMBL4445514 IC50 = 100.0 nM 7.00
CHEMBL3409366 IC50 = 182.0 nM 6.74
CHEMBL3409369 IC50 = 419.0 nM 6.38
CHEMBL4476269 IC50 = 627.0 nM 6.20
CHEMBL4547451 IC50 = 3274.0 nM 5.49
CHEMBL4447350 IC50 = 4636.0 nM 5.33
CHEMBL4553065 IC50 = 6382.0 nM 5.20
CHEMBL4465733 IC50 = 10140.0 nM 4.99
CHEMBL4467988 IC50 = 14260.0 nM 4.85
CHEMBL4570469 IC50 = 15940.0 nM 4.80
CHEMBL4580855 IC50 = 23550.0 nM 4.63
CHEMBL4453241 IC50 = 34000.0 nM 4.47
CHEMBL4592035 IC50 = 35170.0 nM 4.45
CHEMBL4550229 IC50 = 38100.0 nM 4.42
CHEMBL4518301 IC50 = 38700.0 nM 4.41
CHEMBL4471025 IC50 = 40620.0 nM 4.39
CHEMBL4436476 IC50 = 53970.0 nM 4.27
CHEMBL4545610 IC50 = 55100.0 nM 4.26
CHEMBL4564163 IC50 = 71860.0 nM 4.14
CHEMBL4578039 IC50 = 74680.0 nM 4.13
CHEMBL4463496 IC50 = 75190.0 nM 4.12