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Assay Detail

CHEMBL4402446

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of TYK2 JH2 domain in human whole blood assessed as decrease in IFNalpha/IL2-induced STAT phosphorylation preincubated for 1 hr followed by IFNalpha/IL2 stimulation and measured after 15 mins by fluorescence assay
22
Total Activities
22
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Tissue Blood
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Non-receptor tyrosine-protein kinase TYK2 (CHEMBL3553)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Identification of Imidazo[1,2-b]pyridazine Derivatives as Potent, Selective, and Orally Active Tyk2 JH2 Inhibitors.
Liu C, Lin J, Moslin R, Tokarski JS, Muckelbauer J, Chang C, Tredup J, Xie D, Park H, Li P, Wu DR, Strnad J, Zupa-Fernandez A, Cheng L, Chaudhry C, Chen J, Chen C, Sun H, Elzinga P, D'arienzo C, Gillooly K, Taylor TL, McIntyre KW, Salter-Cid L, Lombardo LJ, Carter PH, Aranibar N, Burke JR, Weinstein DS.
ACS Med Chem Lett (2019) 10 : 383 -388
PubMed 30891145 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 22 6.57 7.20

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4442827 1 7.20
CHEMBL4532948 1 7.09
CHEMBL4439957 1 7.07
CHEMBL4460368 1 7.05
CHEMBL4571920 1 6.87
CHEMBL4561123 1 6.63
CHEMBL4443010 1 6.61
CHEMBL4453441 1 6.57
CHEMBL4543066 1 6.54
CHEMBL4435047 1 6.50
CHEMBL4454109 1 6.47
CHEMBL4585272 1 6.46
CHEMBL4579439 1 6.43
CHEMBL4474801 1 6.42
CHEMBL4466139 1 6.42
CHEMBL4438296 1 6.42
CHEMBL4437714 1 6.33
CHEMBL4438107 1 6.12
CHEMBL4434711 1 6.09
CHEMBL4547009 1 6.09
CHEMBL4293907 1 -
CHEMBL4517542 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4442827 Ki = 63.0 nM 7.20
CHEMBL4532948 Ki = 81.0 nM 7.09
CHEMBL4439957 Ki = 85.0 nM 7.07
CHEMBL4460368 Ki = 90.0 nM 7.05
CHEMBL4571920 Ki = 136.0 nM 6.87
CHEMBL4561123 Ki = 236.0 nM 6.63
CHEMBL4443010 Ki = 247.0 nM 6.61
CHEMBL4453441 Ki = 268.0 nM 6.57
CHEMBL4543066 Ki = 285.0 nM 6.54
CHEMBL4435047 Ki = 313.0 nM 6.50
CHEMBL4454109 Ki = 340.0 nM 6.47
CHEMBL4585272 Ki = 346.0 nM 6.46
CHEMBL4579439 Ki = 371.0 nM 6.43
CHEMBL4474801 Ki = 380.0 nM 6.42
CHEMBL4466139 Ki = 382.0 nM 6.42
CHEMBL4438296 Ki = 384.0 nM 6.42
CHEMBL4437714 Ki = 464.0 nM 6.33
CHEMBL4438107 Ki = 750.0 nM 6.12
CHEMBL4434711 Ki = 811.0 nM 6.09
CHEMBL4547009 Ki = 817.0 nM 6.09
CHEMBL4293907 Ki - -
CHEMBL4517542 Ki - -