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Assay Detail

CHEMBL4403931

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human HeLa cell-derived full length DNA-PK catalytic subunit using fluorescein-EPPLSQEAFADLWKK as substrate preincubated for 30 mins followed by substrate addition and measured after 40 mins TR-FRET assay
21
Total Activities
20
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HeLa
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

DNA-dependent protein kinase catalytic subunit (CHEMBL3142)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

The Discovery of 7-Methyl-2-[(7-methyl[1,2,4]triazolo[1,5-<i>a</i>]pyridin-6-yl)amino]-9-(tetrahydro-2<i>H</i>-pyran-4-yl)-7,9-dihydro-8<i>H</i>-purin-8-one (AZD7648), a Potent and Selective DNA-Dependent Protein Kinase (DNA-PK) Inhibitor.
Goldberg FW, Finlay MRV, Ting AKT, Beattie D, Lamont GM, Fallan C, Wrigley GL, Schimpl M, Howard MR, Williamson B, Vazquez-Chantada M, Barratt DG, Davies BR, Cadogan EB, Ramos-Montoya A, Dean E.
J Med Chem (2020) 63 : 3461 -3471
PubMed 31851518 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 21 8.81 10.10

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4447991 1 10.10
CHEMBL4560221 1 9.80
CHEMBL4450232 1 9.70
CHEMBL4553941 1 9.60
CHEMBL4555040 1 9.50
CHEMBL4581254 1 9.50
CHEMBL4513701 1 9.40
CHEMBL4474647 1 9.40
CHEMBL4439259 AZD-7648 1.0 2 9.22
CHEMBL4459057 1 8.70
CHEMBL4438672 1 8.60
CHEMBL4474502 1 8.50
CHEMBL4529390 1 8.30
CHEMBL4483014 1 8.30
CHEMBL4586576 1 8.20
CHEMBL4552812 1 8.10
CHEMBL4466619 1 8.00
CHEMBL4449235 1 8.00
CHEMBL4450430 1 7.90
CHEMBL4456514 1 6.90

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4447991 IC50 = 0.07943 nM 10.10
CHEMBL4560221 IC50 = 0.1585 nM 9.80
CHEMBL4450232 IC50 = 0.1995 nM 9.70
CHEMBL4553941 IC50 = 0.2512 nM 9.60
CHEMBL4555040 IC50 = 0.3162 nM 9.50
CHEMBL4581254 IC50 = 0.3162 nM 9.50
CHEMBL4513701 IC50 = 0.3981 nM 9.40
CHEMBL4474647 IC50 = 0.3981 nM 9.40
CHEMBL4439259 AZD-7648 IC50 = 0.6 nM 9.22
CHEMBL4439259 AZD-7648 IC50 = 0.631 nM 9.20
CHEMBL4459057 IC50 = 1.995 nM 8.70
CHEMBL4438672 IC50 = 2.512 nM 8.60
CHEMBL4474502 IC50 = 3.162 nM 8.50
CHEMBL4529390 IC50 = 5.012 nM 8.30
CHEMBL4483014 IC50 = 5.012 nM 8.30
CHEMBL4586576 IC50 = 6.31 nM 8.20
CHEMBL4552812 IC50 = 7.943 nM 8.10
CHEMBL4466619 IC50 = 10.0 nM 8.00
CHEMBL4449235 IC50 = 10.0 nM 8.00
CHEMBL4450430 IC50 = 12.59 nM 7.90
CHEMBL4456514 IC50 = 125.89 nM 6.90