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Assay Detail

CHEMBL4403932

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of DNA-PK in human A549 cells assessed as reduction in irradiation-induced autophosphorylation at S2056 residue preincubated for 1 hr followed by 8 Gy irradiation and measured after 1 hr by ELISA
18
Total Activities
17
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type A549
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

DNA-dependent protein kinase catalytic subunit (CHEMBL3142)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

The Discovery of 7-Methyl-2-[(7-methyl[1,2,4]triazolo[1,5-<i>a</i>]pyridin-6-yl)amino]-9-(tetrahydro-2<i>H</i>-pyran-4-yl)-7,9-dihydro-8<i>H</i>-purin-8-one (AZD7648), a Potent and Selective DNA-Dependent Protein Kinase (DNA-PK) Inhibitor.
Goldberg FW, Finlay MRV, Ting AKT, Beattie D, Lamont GM, Fallan C, Wrigley GL, Schimpl M, Howard MR, Williamson B, Vazquez-Chantada M, Barratt DG, Davies BR, Cadogan EB, Ramos-Montoya A, Dean E.
J Med Chem (2020) 63 : 3461 -3471
PubMed 31851518 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 18 6.77 7.60

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4560221 1 7.60
CHEMBL4555040 1 7.50
CHEMBL4450232 1 7.30
CHEMBL4447991 1 7.30
CHEMBL4474647 1 7.30
CHEMBL4513701 1 7.20
CHEMBL4439259 AZD-7648 1.0 2 7.04
CHEMBL4581254 1 6.90
CHEMBL4474502 1 6.90
CHEMBL4459057 1 6.80
CHEMBL4449235 1 6.60
CHEMBL4553941 1 6.60
CHEMBL4483014 1 6.50
CHEMBL4438672 1 6.40
CHEMBL4466619 1 5.90
CHEMBL4529390 1 5.70
CHEMBL4456514 1 5.30

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4560221 IC50 = 25.12 nM 7.60
CHEMBL4555040 IC50 = 31.62 nM 7.50
CHEMBL4450232 IC50 = 50.12 nM 7.30
CHEMBL4447991 IC50 = 50.12 nM 7.30
CHEMBL4474647 IC50 = 50.12 nM 7.30
CHEMBL4513701 IC50 = 63.1 nM 7.20
CHEMBL4439259 AZD-7648 IC50 = 91.0 nM 7.04
CHEMBL4439259 AZD-7648 IC50 = 100.0 nM 7.00
CHEMBL4581254 IC50 = 125.89 nM 6.90
CHEMBL4474502 IC50 = 125.89 nM 6.90
CHEMBL4459057 IC50 = 158.49 nM 6.80
CHEMBL4553941 IC50 = 251.19 nM 6.60
CHEMBL4449235 IC50 = 251.19 nM 6.60
CHEMBL4483014 IC50 = 316.23 nM 6.50
CHEMBL4438672 IC50 = 398.11 nM 6.40
CHEMBL4466619 IC50 = 1258.93 nM 5.90
CHEMBL4529390 IC50 = 1995.26 nM 5.70
CHEMBL4456514 IC50 = 5011.87 nM 5.30