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Assay Detail

CHEMBL4404287

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of ClpP in methicillin-resistant Staphylococcus aureus ATCC 33591 assessed as reduction in bacteria-induced hemolysis of sheep erythrocytes by measuring hemoglobin release incubated for 15 mins
7
Total Activities
7
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Staphylococcus aureus
Confidence 8 — Homologous single protein target
Curated By Autocuration

Target

ATP-dependent Clp protease proteolytic subunit (CHEMBL1932910)
Type SINGLE PROTEIN
Organism Staphylococcus aureus (strain NCTC 8325)

Publication

Discovery of Novel Peptidomimetic Boronate ClpP Inhibitors with Noncanonical Enzyme Mechanism as Potent Virulence Blockers in Vitro and in Vivo.
Ju Y, He L, Zhou Y, Yang T, Sun K, Song R, Yang Y, Li C, Sang Z, Bao R, Luo Y.
J Med Chem (2020) 63 : 3104 -3119
PubMed 32031798 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 7 5.26 5.85

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4446834 1 5.85
CHEMBL4540126 1 5.58
CHEMBL4536802 1 5.48
CHEMBL2141296 IXAZOMIB 3.0 1 5.44
CHEMBL4438765 1 5.02
CHEMBL4457363 1 4.83
CHEMBL325041 BORTEZOMIB 3.0 1 4.63

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4446834 EC50 = 1400.0 nM 5.85
CHEMBL4540126 EC50 = 2600.0 nM 5.58
CHEMBL4536802 EC50 = 3300.0 nM 5.48
CHEMBL2141296 IXAZOMIB EC50 = 3600.0 nM 5.44
CHEMBL4438765 EC50 = 9600.0 nM 5.02
CHEMBL4457363 EC50 = 14800.0 nM 4.83
CHEMBL325041 BORTEZOMIB EC50 = 23500.0 nM 4.63