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Assay Detail

CHEMBL4405928

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of BRD4 in poly(I:C)-stimulated human SAECs TLR3-inducible IL-6 gene expression pre-incubated for 24 hrs before poly(I:C) stimulation for 4 hrs by qRT-PCR analysis
9
Total Activities
9
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Bromodomain-containing protein 4 (CHEMBL1163125)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of Orally Bioavailable Chromone Derivatives as Potent and Selective BRD4 Inhibitors: Scaffold Hopping, Optimization, and Pharmacological Evaluation.
Liu Z, Chen H, Wang P, Li Y, Wold EA, Leonard PG, Joseph S, Brasier AR, Tian B, Zhou J.
J Med Chem (2020) 63 : 5242 -5256
PubMed 32255647 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 9 5.93 6.51

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4557050 1 6.51
CHEMBL4549898 1 6.27
CHEMBL4517875 1 6.12
CHEMBL4453427 1 6.01
CHEMBL1957266 1 5.99
CHEMBL4455209 1 5.96
CHEMBL4543985 1 5.55
CHEMBL2393130 APABETALONE 3.0 1 5.48
CHEMBL4555828 1 5.46

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4557050 IC50 = 310.0 nM 6.51
CHEMBL4549898 IC50 = 540.0 nM 6.27
CHEMBL4517875 IC50 = 750.0 nM 6.12
CHEMBL4453427 IC50 = 970.0 nM 6.01
CHEMBL1957266 IC50 = 1020.0 nM 5.99
CHEMBL4455209 IC50 = 1100.0 nM 5.96
CHEMBL4543985 IC50 = 2800.0 nM 5.55
CHEMBL2393130 APABETALONE IC50 = 3290.0 nM 5.48
CHEMBL4555828 IC50 = 3500.0 nM 5.46