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Assay Detail

CHEMBL4407115

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of ATG4B (unknown origin) using His-GATE-16-GST as substrate preincubated for 30 mins followed by substrate addition measured after 30 mins by TR-FRET assay
1
Total Activities
1
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Cysteine protease ATG4B (CHEMBL1741221)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Identification of benzo[cd]indol-2(1H)-ones as novel Atg4B inhibitors via a structure-based virtual screening and a novel AlphaScreen assay.
Quintana M, Bilbao A, Comas-Barceló J, Bujons J, Triola G.
Eur J Med Chem (2019) 178 : 648 -666
PubMed 31226656 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 1 6.44 6.44

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4469737 1 6.44

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4469737 IC50 = 360.0 nM 6.44