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Assay Detail

CHEMBL4407126

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Antagonist activity at PAR4 in human washed platelets assessed as inhibition of gamma-thrombin-induced platelet aggregation preincubated for 5 mins followed by gamma-thrombin addition and measured every 50 secs for up to 15 mins starting from 10 secs post gamma-thrombin addition in presence of recombinant hirudin
22
Total Activities
22
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Proteinase-activated receptor 4 (CHEMBL4691)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of Potent Protease-Activated Receptor 4 Antagonists with in Vivo Antithrombotic Efficacy.
Miller MM, Banville J, Friends TJ, Gagnon M, Hangeland JJ, Lavallée JF, Martel A, O'Grady H, Rémillard R, Ruediger E, Tremblay F, Posy SL, Allegretto NJ, Guarino VR, Harden DG, Harper TW, Hartl K, Josephs J, Malmstrom S, Watson C, Yang Y, Zhang G, Wong P, Yang J, Bouvier M, Seiffert DA, Wexler RR, Lawrence RM, Priestley ES, Marinier A.
J Med Chem (2019) 62 : 7400 -7416
PubMed 31246024 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 22 6.36 8.10

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3728620 1 8.10
CHEMBL3728724 1 7.13
CHEMBL3728462 1 6.92
CHEMBL3732386 1 6.62
CHEMBL3931873 1 6.62
CHEMBL3728507 1 6.57
CHEMBL4439280 1 6.46
CHEMBL3732490 1 6.00
CHEMBL3731720 1 5.75
CHEMBL3727598 1 5.68
CHEMBL3732510 1 5.68
CHEMBL3729282 1 5.62
CHEMBL3730957 1 5.51
CHEMBL3728010 1 -
CHEMBL4471641 1 -
CHEMBL4444227 1 -
CHEMBL4435447 1 -
CHEMBL4572935 1 -
CHEMBL3730643 1 -
CHEMBL3728130 1 -
CHEMBL3731871 1 -
CHEMBL3732594 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3728620 IC50 = 8.0 nM 8.10
CHEMBL3728724 IC50 = 74.0 nM 7.13
CHEMBL3728462 IC50 = 120.0 nM 6.92
CHEMBL3732386 IC50 = 240.0 nM 6.62
CHEMBL3931873 IC50 = 240.0 nM 6.62
CHEMBL3728507 IC50 = 270.0 nM 6.57
CHEMBL4439280 IC50 = 350.0 nM 6.46
CHEMBL3732490 IC50 = 1000.0 nM 6.00
CHEMBL3731720 IC50 = 1800.0 nM 5.75
CHEMBL3727598 IC50 = 2100.0 nM 5.68
CHEMBL3732510 IC50 = 2100.0 nM 5.68
CHEMBL3729282 IC50 = 2400.0 nM 5.62
CHEMBL3730957 IC50 = 3100.0 nM 5.51
CHEMBL3728010 IC50 > 30000.0 nM -
CHEMBL4471641 IC50 > 30000.0 nM -
CHEMBL4444227 IC50 - -
CHEMBL4435447 IC50 - -
CHEMBL4572935 IC50 > 30000.0 nM -
CHEMBL3730643 IC50 > 30000.0 nM -
CHEMBL3728130 IC50 > 30000.0 nM -
CHEMBL3731871 IC50 > 30000.0 nM -
CHEMBL3732594 IC50 > 30000.0 nM -