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Assay Detail

CHEMBL4407783

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of N-terminal unlinked Zika virus NS2B (45 to 96 residues) - NS3 (1 to 177 residues) protease domain expressed in Escherichia coli Bl21(DE3) using Bz-nKRR-AMC as substrate preincubated for 15 mins followed by substrate addition and measured by fluorescence based assay
16
Total Activities
16
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Zika virus
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Nonstructural protein 3 (CHEMBL4523954)
Type SINGLE PROTEIN
Organism Zika virus

Publication

Cell-active carbazole derivatives as inhibitors of the zika virus protease.
Rassias G, Zogali V, Swarbrick CMD, Ki Chan KW, Chan SA, Gwee CP, Wang S, Kaplanai E, Canko A, Kiousis D, Lescar J, Luo D, Matsoukas MT, Vasudevan SG.
Eur J Med Chem (2019) 180 : 536 -545
PubMed 31344613 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 16 5.29 6.28

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4448497 1 6.28
CHEMBL4558642 1 6.09
CHEMBL4568823 1 6.00
CHEMBL4437288 1 5.77
CHEMBL4446364 1 5.76
CHEMBL4447165 1 5.72
CHEMBL4518903 1 5.46
CHEMBL4532866 1 5.39
CHEMBL4548788 1 5.09
CHEMBL4457232 1 5.04
CHEMBL4470866 1 4.86
CHEMBL4437316 1 4.85
CHEMBL4535925 1 4.83
CHEMBL4444928 1 4.59
CHEMBL4544053 1 4.49
CHEMBL3040170 1 4.42

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4448497 IC50 = 520.0 nM 6.28
CHEMBL4558642 IC50 = 820.0 nM 6.09
CHEMBL4568823 IC50 = 990.0 nM 6.00
CHEMBL4437288 IC50 = 1700.0 nM 5.77
CHEMBL4446364 IC50 = 1750.0 nM 5.76
CHEMBL4447165 IC50 = 1910.0 nM 5.72
CHEMBL4518903 IC50 = 3500.0 nM 5.46
CHEMBL4532866 IC50 = 4040.0 nM 5.39
CHEMBL4548788 IC50 = 8100.0 nM 5.09
CHEMBL4457232 IC50 = 9190.0 nM 5.04
CHEMBL4470866 IC50 = 13860.0 nM 4.86
CHEMBL4437316 IC50 = 14150.0 nM 4.85
CHEMBL4535925 IC50 = 14940.0 nM 4.83
CHEMBL4444928 IC50 = 25600.0 nM 4.59
CHEMBL4544053 IC50 = 32520.0 nM 4.49
CHEMBL3040170 IC50 = 38275.0 nM 4.42