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Assay Detail

CHEMBL4408248

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Positive allosteric modulation of recombinant human GluN1/GluN2A receptor stably expressed in HEK293 cells assessed as increase in glycine/L-glutamate-induced channel current at -70 mV holding potential by IonWork two-electrode voltage clamp method
27
Total Activities
27
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 7 — Homologous single protein target
Curated By Autocuration

Target

Glutamate NMDA receptor; GRIN1/GRIN2A (CHEMBL1907604)
Type PROTEIN COMPLEX
Organism Homo sapiens

Publication

Neuroactive Steroid <i>N</i>-Methyl-d-aspartate Receptor Positive Allosteric Modulators: Synthesis, SAR, and Pharmacological Activity.
La DS, Salituro FG, Martinez Botella G, Griffin AM, Bai Z, Ackley MA, Dai J, Doherty JJ, Harrison BL, Hoffmann EC, Kazdoba TM, Lewis MC, Quirk MC, Robichaud AJ.
J Med Chem (2019) 62 : 7526 -7542
PubMed 31390523 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 27 6.73 7.52

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4555750 1 7.52
CHEMBL4516765 1 7.48
CHEMBL170154 1 7.43
CHEMBL4513977 1 7.11
CHEMBL4517382 1 7.10
CHEMBL4450472 1 7.00
CHEMBL4550075 1 6.92
CHEMBL4586043 1 6.92
CHEMBL171804 1 6.82
CHEMBL2419157 1 6.75
CHEMBL4475011 1 6.66
CHEMBL4529930 1 6.57
CHEMBL4567377 1 6.50
CHEMBL4464591 1 6.50
CHEMBL4468040 1 6.38
CHEMBL4448829 1 6.32
CHEMBL4439636 1 6.26
CHEMBL4464781 1 6.05
CHEMBL2419158 1 5.52
CHEMBL4521106 1 -
CHEMBL4476306 1 -
CHEMBL4548602 1 -
CHEMBL4522435 1 -
CHEMBL4573975 1 -
CHEMBL169046 1 -
CHEMBL4579269 1 -
CHEMBL4446143 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4555750 EC50 = 30.0 nM 7.52
CHEMBL4516765 EC50 = 33.0 nM 7.48
CHEMBL170154 EC50 = 37.0 nM 7.43
CHEMBL4513977 EC50 = 77.0 nM 7.11
CHEMBL4517382 EC50 = 80.0 nM 7.10
CHEMBL4450472 EC50 = 100.0 nM 7.00
CHEMBL4550075 EC50 = 120.0 nM 6.92
CHEMBL4586043 EC50 = 120.0 nM 6.92
CHEMBL171804 EC50 = 150.0 nM 6.82
CHEMBL2419157 EC50 = 180.0 nM 6.75
CHEMBL4475011 EC50 = 220.0 nM 6.66
CHEMBL4529930 EC50 = 270.0 nM 6.57
CHEMBL4567377 EC50 = 320.0 nM 6.50
CHEMBL4464591 EC50 = 320.0 nM 6.50
CHEMBL4468040 EC50 = 420.0 nM 6.38
CHEMBL4448829 EC50 = 480.0 nM 6.32
CHEMBL4439636 EC50 = 550.0 nM 6.26
CHEMBL4464781 EC50 = 900.0 nM 6.05
CHEMBL2419158 EC50 = 3000.0 nM 5.52
CHEMBL4521106 EC50 > 10000.0 nM -
CHEMBL4476306 EC50 > 10000.0 nM -
CHEMBL4548602 EC50 > 10000.0 nM -
CHEMBL4522435 EC50 > 10000.0 nM -
CHEMBL4573975 EC50 > 10000.0 nM -
CHEMBL169046 EC50 > 10000.0 nM -
CHEMBL4579269 EC50 > 10000.0 nM -
CHEMBL4446143 EC50 > 10000.0 nM -