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Assay Detail

CHEMBL4409078

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of FAAH in mouse brain membranes assessed as inhibitory constant using [14C]-AEA as substrate incubated for 15 mins by scintillation counting method
10
Total Activities
10
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Mus musculus
Tissue Brain
Subcellular Membranes
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Fatty-acid amide hydrolase 1 (CHEMBL3455)
Type SINGLE PROTEIN
Organism Mus musculus

Publication

Development of novel multipotent compounds modulating endocannabinoid and dopaminergic systems.
Grillo A, Chemi G, Brogi S, Brindisi M, Relitti N, Fezza F, Fazio D, Castelletti L, Perdona E, Wong A, Lamponi S, Pecorelli A, Benedusi M, Fantacci M, Valoti M, Valacchi G, Micheli F, Novellino E, Campiani G, Butini S, Maccarrone M, Gemma S.
Eur J Med Chem (2019) 183 : 111674 -111674
PubMed 31518969 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 10 7.54 9.64

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4471658 1 9.64
CHEMBL4565200 1 8.16
CHEMBL4473519 1 7.80
CHEMBL4540218 1 7.80
CHEMBL4554053 1 7.60
CHEMBL4520982 1 7.34
CHEMBL4449756 1 7.30
CHEMBL4520184 1 6.71
CHEMBL4517296 1 6.69
CHEMBL4446507 1 6.38

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4471658 Ki = 0.23 nM 9.64
CHEMBL4565200 Ki = 6.88 nM 8.16
CHEMBL4473519 Ki = 16.0 nM 7.80
CHEMBL4540218 Ki = 16.0 nM 7.80
CHEMBL4554053 Ki = 25.0 nM 7.60
CHEMBL4520982 Ki = 46.0 nM 7.34
CHEMBL4449756 Ki = 50.0 nM 7.30
CHEMBL4520184 Ki = 194.0 nM 6.71
CHEMBL4517296 Ki = 203.0 nM 6.69
CHEMBL4446507 Ki = 420.0 nM 6.38