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Assay Detail

CHEMBL4410890

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of EGFR L858R mutant (unknown origin) expressed in mouse Ba/F3 cells assessed as reduction in cell growth incubated for 72 hrs in presence of 1 ug/ml Cetuximab by celltiter-glo luminescent cell viability assay
5
Total Activities
5
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Epidermal growth factor receptor (CHEMBL203)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery and Optimization of Dibenzodiazepinones as Allosteric Mutant-Selective EGFR Inhibitors.
De Clercq DJH, Heppner DE, To C, Jang J, Park E, Yun CH, Mushajiang M, Shin BH, Gero TW, Scott DA, Jänne PA, Eck MJ, Gray NS.
ACS Med Chem Lett (2019) 10 : 1549 -1553
PubMed 31749909 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 5 5.63 6.08

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4452106 1 6.08
CHEMBL4558528 1 5.57
CHEMBL4533337 1 5.43
CHEMBL4530686 1 5.42
CHEMBL4520903 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4452106 IC50 = 840.0 nM 6.08
CHEMBL4558528 IC50 = 2700.0 nM 5.57
CHEMBL4533337 IC50 = 3700.0 nM 5.43
CHEMBL4530686 IC50 = 3800.0 nM 5.42
CHEMBL4520903 IC50 > 10000.0 nM -