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Assay Detail

CHEMBL4411114

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of PREP in C57BL/6 mouse cortex homogenate assessed as formation of 7-amido-4-methylcoumarin using Suc-Gly-Pro-amido-4-methylcoumarin as substrate preincubated for 30 mins followed by substrate addition measured after 60 mins by fluorometric assay
13
Total Activities
13
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Mus musculus
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Prolyl endopeptidase (CHEMBL4935)
Type SINGLE PROTEIN
Organism Mus musculus

Publication

Tetrazole as a Replacement of the Electrophilic Group in Characteristic Prolyl Oligopeptidase Inhibitors.
Kilpeläinen TP, Tyni JK, Lahtela-Kakkonen MK, Eteläinen TS, Myöhänen TT, Wallén EAA.
ACS Med Chem Lett (2019) 10 : 1635 -1640
PubMed 31857839 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 13 6.13 9.52

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL189620 1 9.52
CHEMBL4549821 1 8.48
CHEMBL4528594 1 7.04
CHEMBL4465322 1 6.58
CHEMBL4556841 1 6.53
CHEMBL4540171 1 5.29
CHEMBL4588059 1 5.21
CHEMBL4586782 1 4.92
CHEMBL4520738 1 4.87
CHEMBL4475037 1 4.54
CHEMBL4551013 1 4.45
CHEMBL4441906 1 -
CHEMBL4436952 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL189620 IC50 = 0.3 nM 9.52
CHEMBL4549821 IC50 = 3.28 nM 8.48
CHEMBL4528594 IC50 = 91.0 nM 7.04
CHEMBL4465322 IC50 = 264.0 nM 6.58
CHEMBL4556841 IC50 = 298.0 nM 6.53
CHEMBL4540171 IC50 = 5141.0 nM 5.29
CHEMBL4588059 IC50 = 6213.0 nM 5.21
CHEMBL4586782 IC50 = 12080.0 nM 4.92
CHEMBL4520738 IC50 = 13510.0 nM 4.87
CHEMBL4475037 IC50 = 28800.0 nM 4.54
CHEMBL4551013 IC50 = 35180.0 nM 4.45
CHEMBL4441906 IC50 = 153800.0 nM -
CHEMBL4436952 IC50 = 457000.0 nM -