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Assay Detail

CHEMBL4416105

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of GST-tagged human EGFR kinase domain (696 to 1022 residues) using poly(Glu, Tyr) 4:1 substrate incubated for 60 mins by kinase-Glo plus luminescent kinase assay
26
Total Activities
26
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Epidermal growth factor receptor (CHEMBL203)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of a Furanopyrimidine-Based Epidermal Growth Factor Receptor Inhibitor (DBPR112) as a Clinical Candidate for the Treatment of Non-Small Cell Lung Cancer.
Lin SY, Chang Hsu Y, Peng YH, Ke YY, Lin WH, Sun HY, Shiao HY, Kuo FM, Chen PY, Lien TW, Chen CH, Chu CY, Wang SY, Yeh KC, Chen CP, Hsu TA, Wu SY, Yeh TK, Chen CT, Hsieh HP.
J Med Chem (2019) 62 : 10108 -10123
PubMed 31560541 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 26 6.96 7.89

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4458437 1 7.89
CHEMBL4463012 1 7.85
CHEMBL1173655 AFATINIB 4.0 1 7.85
CHEMBL4521381 1 7.82
CHEMBL4583178 1 7.72
CHEMBL1172843 1 7.70
CHEMBL4559807 1 7.62
CHEMBL939 GEFITINIB 4.0 1 7.54
CHEMBL4448691 1 7.21
CHEMBL4456359 1 7.19
CHEMBL4446953 1 7.10
CHEMBL4556670 1 7.09
CHEMBL4475546 1 7.09
CHEMBL4447962 1 6.97
CHEMBL4514452 1 6.93
CHEMBL4534719 1 6.87
CHEMBL4441440 1 6.80
CHEMBL1172781 1 6.65
CHEMBL4448148 1 6.62
CHEMBL2310642 1 6.62
CHEMBL4447289 1 6.38
CHEMBL4450843 1 6.25
CHEMBL4565374 1 5.51
CHEMBL4447036 1 5.43
CHEMBL4527132 1 5.30
CHEMBL4531827 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4458437 IC50 = 13.0 nM 7.89
CHEMBL4463012 IC50 = 14.0 nM 7.85
CHEMBL1173655 AFATINIB IC50 = 14.0 nM 7.85
CHEMBL4521381 IC50 = 15.0 nM 7.82
CHEMBL4583178 IC50 = 19.0 nM 7.72
CHEMBL1172843 IC50 = 20.0 nM 7.70
CHEMBL4559807 IC50 = 24.0 nM 7.62
CHEMBL939 GEFITINIB IC50 = 29.0 nM 7.54
CHEMBL4448691 IC50 = 62.0 nM 7.21
CHEMBL4456359 IC50 = 64.0 nM 7.19
CHEMBL4446953 IC50 = 79.0 nM 7.10
CHEMBL4556670 IC50 = 82.0 nM 7.09
CHEMBL4475546 IC50 = 81.0 nM 7.09
CHEMBL4447962 IC50 = 107.0 nM 6.97
CHEMBL4514452 IC50 = 117.0 nM 6.93
CHEMBL4534719 IC50 = 136.0 nM 6.87
CHEMBL4441440 IC50 = 159.0 nM 6.80
CHEMBL1172781 IC50 = 223.0 nM 6.65
CHEMBL4448148 IC50 = 239.0 nM 6.62
CHEMBL2310642 IC50 = 238.0 nM 6.62
CHEMBL4447289 IC50 = 416.0 nM 6.38
CHEMBL4450843 IC50 = 562.0 nM 6.25
CHEMBL4565374 IC50 = 3059.0 nM 5.51
CHEMBL4447036 IC50 = 3675.0 nM 5.43
CHEMBL4527132 IC50 = 5004.0 nM 5.30
CHEMBL4531827 IC50 > 10000.0 nM -