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Assay Detail

CHEMBL4416568

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Agonist activity at beta2 adrenergic receptor in guinea pig tracheal smooth muscle assessed as inhibition of histamine-induced tracheal smooth muscle contraction by measuring spasmolysis preincubated for 15 mins in presence of beta adrenergic receptor antagonist propranolol followed by histamine addition
5
Total Activities
3
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Cavia porcellus
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Beta-2 adrenergic receptor (CHEMBL5414)
Type SINGLE PROTEIN
Organism Cavia porcellus

Publication

Synthesis, Purification, and Selective β2-AR Agonist and Bronchodilatory Effects of Catecholic Tetrahydroisoquinolines from Portulaca oleracea.
Yang EL, Sun B, Huang ZY, Lin JG, Jiao B, Xiang L.
J Nat Prod (2019) 82 : 2986 -2993
PubMed 31625751 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 3 6.58 8.10
Inhibition 2 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL434 ISOPROTERENOL 4.0 1 8.10
CHEMBL298285 2 6.10
CHEMBL4438390 2 5.55

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL434 ISOPROTERENOL EC50 = 7.9 nM 8.10
CHEMBL298285 EC50 = 800.0 nM 6.10
CHEMBL4438390 EC50 = 2800.0 nM 5.55
CHEMBL4438390 Inhibition - % -
CHEMBL298285 Inhibition - % -