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Assay Detail

CHEMBL4416608

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of NLRP3 in mouse J774A.1 cells assessed as inhibition of LPS and ATP-induced IL-1beta production pre-stimulated with LPS for 4.5 hrs before compound addition with ATP for 30 mins by ELISA
27
Total Activities
27
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Mus musculus
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

NACHT, LRR and PYD domains-containing protein 3 (CHEMBL3779755)
Type SINGLE PROTEIN
Organism Mus musculus

Publication

Discovery of Second-Generation NLRP3 Inflammasome Inhibitors: Design, Synthesis, and Biological Characterization.
Jiang Y, He L, Green J, Blevins H, Guo C, Patel SH, Halquist MS, McRae M, Venitz J, Wang XY, Zhang S.
J Med Chem (2019) 62 : 9718 -9731
PubMed 31626545 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 27 6.09 6.52

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4559751 1 6.52
CHEMBL4565429 1 6.51
CHEMBL4570220 1 6.34
CHEMBL4438030 1 6.33
CHEMBL4469246 1 6.33
CHEMBL4459483 1 6.26
CHEMBL4437178 1 6.24
CHEMBL4521108 1 6.21
CHEMBL4543196 1 6.17
CHEMBL4449341 1 6.16
CHEMBL4520880 1 6.14
CHEMBL4536818 1 6.11
CHEMBL4454491 1 6.10
CHEMBL4567140 1 6.06
CHEMBL4456669 1 6.06
CHEMBL4444873 1 6.05
CHEMBL4457339 1 6.05
CHEMBL4469806 1 6.04
CHEMBL4460689 1 6.01
CHEMBL4472886 1 5.97
CHEMBL4445049 1 5.94
CHEMBL4455922 1 5.89
CHEMBL4471456 1 5.83
CHEMBL4537830 1 5.83
CHEMBL4457173 1 5.78
CHEMBL4465138 1 5.75
CHEMBL4568669 1 5.71

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4559751 IC50 = 300.0 nM 6.52
CHEMBL4565429 IC50 = 310.0 nM 6.51
CHEMBL4570220 IC50 = 460.0 nM 6.34
CHEMBL4438030 IC50 = 470.0 nM 6.33
CHEMBL4469246 IC50 = 470.0 nM 6.33
CHEMBL4459483 IC50 = 550.0 nM 6.26
CHEMBL4437178 IC50 = 580.0 nM 6.24
CHEMBL4521108 IC50 = 620.0 nM 6.21
CHEMBL4543196 IC50 = 670.0 nM 6.17
CHEMBL4449341 IC50 = 690.0 nM 6.16
CHEMBL4520880 IC50 = 730.0 nM 6.14
CHEMBL4536818 IC50 = 780.0 nM 6.11
CHEMBL4454491 IC50 = 790.0 nM 6.10
CHEMBL4567140 IC50 = 870.0 nM 6.06
CHEMBL4456669 IC50 = 880.0 nM 6.06
CHEMBL4444873 IC50 = 890.0 nM 6.05
CHEMBL4457339 IC50 = 900.0 nM 6.05
CHEMBL4469806 IC50 = 910.0 nM 6.04
CHEMBL4460689 IC50 = 970.0 nM 6.01
CHEMBL4472886 IC50 = 1060.0 nM 5.97
CHEMBL4445049 IC50 = 1150.0 nM 5.94
CHEMBL4455922 IC50 = 1300.0 nM 5.89
CHEMBL4471456 IC50 = 1490.0 nM 5.83
CHEMBL4537830 IC50 = 1470.0 nM 5.83
CHEMBL4457173 IC50 = 1660.0 nM 5.78
CHEMBL4465138 IC50 = 1780.0 nM 5.75
CHEMBL4568669 IC50 = 1930.0 nM 5.71