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Assay Detail

CHEMBL4417038

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant human N-terminal His-tagged MetAP2 (2 to 478 residues) using Met-Ala-Ser as substrate and MnCl2 as co-facor preincubated for 15 mins followed by substrate addition and measured after 45 mins by AAO/horse radish peroxidase enzyme coupled assay
26
Total Activities
26
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Methionine aminopeptidase 2 (CHEMBL3922)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Identification of Methionine Aminopeptidase-2 (MetAP-2) Inhibitor M8891: A Clinical Compound for the Treatment of Cancer.
Heinrich T, Seenisamy J, Becker F, Blume B, Bomke J, Dietz M, Eckert U, Friese-Hamim M, Gunera J, Hansen K, Leuthner B, Musil D, Pfalzgraf J, Rohdich F, Siegl C, Spuck D, Wegener A, Zenke FT.
J Med Chem (2019) 62 : 11119 -11134
PubMed 31725285 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 26 7.15 7.47

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4454587 1 7.47
CHEMBL4445117 1 7.39
CHEMBL4551283 1 7.36
CHEMBL4460950 1 7.28
CHEMBL4547442 1 7.28
CHEMBL4522216 1 7.28
CHEMBL4464946 1 7.27
CHEMBL4550096 1 7.24
CHEMBL4463138 1 7.23
CHEMBL4570368 1 7.21
CHEMBL4448724 1 7.20
CHEMBL4460444 1 7.19
CHEMBL4448427 1 7.15
CHEMBL4572028 1 7.13
CHEMBL4475680 1 7.12
CHEMBL4458906 1 7.10
CHEMBL4450159 1 7.10
CHEMBL4451402 1 7.06
CHEMBL4591797 1 7.04
CHEMBL4566917 1 7.03
CHEMBL4441643 1 7.02
CHEMBL4553094 1 7.00
CHEMBL4470242 1 6.96
CHEMBL4473359 1 6.96
CHEMBL4444681 1 6.92
CHEMBL4575412 1 6.80

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4454587 IC50 = 34.0 nM 7.47
CHEMBL4445117 IC50 = 41.0 nM 7.39
CHEMBL4551283 IC50 = 44.0 nM 7.36
CHEMBL4460950 IC50 = 52.0 nM 7.28
CHEMBL4547442 IC50 = 53.0 nM 7.28
CHEMBL4522216 IC50 = 52.0 nM 7.28
CHEMBL4464946 IC50 = 54.0 nM 7.27
CHEMBL4550096 IC50 = 58.0 nM 7.24
CHEMBL4463138 IC50 = 59.0 nM 7.23
CHEMBL4570368 IC50 = 62.0 nM 7.21
CHEMBL4448724 IC50 = 63.0 nM 7.20
CHEMBL4460444 IC50 = 64.0 nM 7.19
CHEMBL4448427 IC50 = 71.0 nM 7.15
CHEMBL4572028 IC50 = 74.0 nM 7.13
CHEMBL4475680 IC50 = 75.0 nM 7.12
CHEMBL4458906 IC50 = 79.0 nM 7.10
CHEMBL4450159 IC50 = 80.0 nM 7.10
CHEMBL4451402 IC50 = 87.0 nM 7.06
CHEMBL4591797 IC50 = 92.0 nM 7.04
CHEMBL4566917 IC50 = 94.0 nM 7.03
CHEMBL4441643 IC50 = 95.0 nM 7.02
CHEMBL4553094 IC50 = 100.0 nM 7.00
CHEMBL4470242 IC50 = 110.0 nM 6.96
CHEMBL4473359 IC50 = 110.0 nM 6.96
CHEMBL4444681 IC50 = 120.0 nM 6.92
CHEMBL4575412 IC50 = 160.0 nM 6.80