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Assay Detail

CHEMBL4418244

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of FTO (unknown origin) assessed as reduction in demethylation using 39nt-m6 A-ssDNA and 2OG incubated for 2 hrs by non-denaturing polyacrylamide electrophoresis method
27
Total Activities
27
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Alpha-ketoglutarate-dependent dioxygenase FTO (CHEMBL2331065)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Use of 2-(substituted phenylamino)benzoic acid fto inhibitor in treating leukemia
(2018)

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 27 5.01 7.22

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4572939 1 7.22
CHEMBL4532629 1 6.40
CHEMBL4563024 1 6.16
CHEMBL4442310 1 5.96
CHEMBL4563561 1 5.94
CHEMBL4471728 1 5.92
CHEMBL4567838 1 5.80
CHEMBL4521076 1 5.34
CHEMBL4559749 1 5.12
CHEMBL4460597 1 4.52
CHEMBL4474254 1 4.52
CHEMBL4453402 1 4.52
CHEMBL4517902 1 4.52
CHEMBL4435677 1 4.52
CHEMBL4576909 1 4.52
CHEMBL4563246 1 4.52
CHEMBL4576737 1 4.52
CHEMBL4471543 1 4.52
CHEMBL4470475 1 4.52
CHEMBL4561283 1 4.52
CHEMBL4522884 1 4.52
CHEMBL4446803 1 4.52
CHEMBL4436454 1 4.52
CHEMBL4436398 1 4.52
CHEMBL4452472 1 4.52
CHEMBL4560665 1 4.52
CHEMBL4463988 1 4.52

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4572939 IC50 = 60.0 nM 7.22
CHEMBL4532629 IC50 = 400.0 nM 6.40
CHEMBL4563024 IC50 = 700.0 nM 6.16
CHEMBL4442310 IC50 = 1100.0 nM 5.96
CHEMBL4563561 IC50 = 1140.0 nM 5.94
CHEMBL4471728 IC50 = 1200.0 nM 5.92
CHEMBL4567838 IC50 = 1600.0 nM 5.80
CHEMBL4521076 IC50 = 4600.0 nM 5.34
CHEMBL4559749 IC50 = 7600.0 nM 5.12
CHEMBL4460597 IC50 = 30000.0 nM 4.52
CHEMBL4474254 IC50 = 30000.0 nM 4.52
CHEMBL4453402 IC50 = 30000.0 nM 4.52
CHEMBL4517902 IC50 = 30000.0 nM 4.52
CHEMBL4435677 IC50 = 30000.0 nM 4.52
CHEMBL4576909 IC50 = 30000.0 nM 4.52
CHEMBL4563246 IC50 = 30000.0 nM 4.52
CHEMBL4576737 IC50 = 30000.0 nM 4.52
CHEMBL4471543 IC50 = 30000.0 nM 4.52
CHEMBL4470475 IC50 = 30000.0 nM 4.52
CHEMBL4561283 IC50 = 30000.0 nM 4.52
CHEMBL4522884 IC50 = 30000.0 nM 4.52
CHEMBL4446803 IC50 = 30000.0 nM 4.52
CHEMBL4436454 IC50 = 30000.0 nM 4.52
CHEMBL4436398 IC50 = 30000.0 nM 4.52
CHEMBL4452472 IC50 = 30000.0 nM 4.52
CHEMBL4560665 IC50 = 30000.0 nM 4.52
CHEMBL4463988 IC50 = 30000.0 nM 4.52