Assay Detail
Binding
CHEMBL4418451
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Inhibition of TEV-cleavable hexa-histidine tagged USP28 (1 to 1077 residues) (unknown origin) using ubiquitin-rhodamine 110 as substrate preincubated for 30 mins followed by substrate addition and measured after 20 mins by fluorescence based assay
21
Total Activities
21
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Ubiquitin carboxyl-terminal hydrolase 28 (CHEMBL2157853) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
Thienopyrazine carboxamides as ubiquitin-specific protease inhibitors
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 21 | 6.33 | 6.70 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL4585832 | — | — | 1 | 6.70 |
| CHEMBL4459980 | — | — | 1 | 6.70 |
| CHEMBL4587318 | — | — | 1 | 6.70 |
| CHEMBL4462420 | — | — | 1 | 6.70 |
| CHEMBL4160957 | — | — | 1 | 6.70 |
| CHEMBL4166283 | — | — | 1 | 6.70 |
| CHEMBL4451295 | — | — | 1 | 6.70 |
| CHEMBL4584152 | — | — | 1 | 6.70 |
| CHEMBL4443504 | — | — | 1 | 6.70 |
| CHEMBL4162425 | — | — | 1 | 6.70 |
| CHEMBL4440956 | — | — | 1 | 5.70 |
| CHEMBL4533281 | — | — | 1 | 5.70 |
| CHEMBL4176483 | — | — | 1 | 5.70 |
| CHEMBL4571015 | — | — | 1 | 5.70 |
| CHEMBL4452295 | — | — | 1 | 5.70 |
| CHEMBL4566674 | — | — | 1 | 5.70 |
| CHEMBL4300392 | — | — | 1 | - |
| CHEMBL4444579 | — | — | 1 | - |
| CHEMBL4169696 | — | — | 1 | - |
| CHEMBL4448281 | — | — | 1 | - |
| CHEMBL4173102 | — | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL4585832 | — | IC50 | = | 200.0 | nM | 6.70 |
| CHEMBL4459980 | — | IC50 | = | 200.0 | nM | 6.70 |
| CHEMBL4587318 | — | IC50 | = | 200.0 | nM | 6.70 |
| CHEMBL4462420 | — | IC50 | = | 200.0 | nM | 6.70 |
| CHEMBL4160957 | — | IC50 | = | 200.0 | nM | 6.70 |
| CHEMBL4166283 | — | IC50 | = | 200.0 | nM | 6.70 |
| CHEMBL4451295 | — | IC50 | = | 200.0 | nM | 6.70 |
| CHEMBL4584152 | — | IC50 | = | 200.0 | nM | 6.70 |
| CHEMBL4443504 | — | IC50 | = | 200.0 | nM | 6.70 |
| CHEMBL4162425 | — | IC50 | = | 200.0 | nM | 6.70 |
| CHEMBL4440956 | — | IC50 | = | 2000.0 | nM | 5.70 |
| CHEMBL4533281 | — | IC50 | = | 2000.0 | nM | 5.70 |
| CHEMBL4176483 | — | IC50 | = | 2000.0 | nM | 5.70 |
| CHEMBL4571015 | — | IC50 | = | 2000.0 | nM | 5.70 |
| CHEMBL4452295 | — | IC50 | = | 2000.0 | nM | 5.70 |
| CHEMBL4566674 | — | IC50 | = | 2000.0 | nM | 5.70 |
| CHEMBL4300392 | — | IC50 | < | 200.0 | nM | - |
| CHEMBL4444579 | — | IC50 | < | 200.0 | nM | - |
| CHEMBL4169696 | — | IC50 | < | 200.0 | nM | - |
| CHEMBL4448281 | — | IC50 | < | 200.0 | nM | - |
| CHEMBL4173102 | — | IC50 | < | 200.0 | nM | - |