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Assay Detail

CHEMBL4420247

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of RPE65 in bovine RPE microsomes assessed as reduction in isomero-hydrolase activity using all-trans-[3H]-retinol by normal phase HPLC with flow scintillation analysis
11
Total Activities
11
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Bos taurus
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Retinoid isomerohydrolase (CHEMBL4523306)
Type SINGLE PROTEIN
Organism Bos taurus

Publication

Compositions and methods of inhibiting retinal degeneration
(2013)

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 11 4.15 4.30

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4460257 1 4.30
CHEMBL4526803 1 4.22
CHEMBL4563763 1 4.22
CHEMBL13210 1 4.00
CHEMBL539219 1 4.00
CHEMBL4456172 1 -
CHEMBL4561265 1 -
CHEMBL4468736 1 -
CHEMBL4554552 1 -
CHEMBL4445639 1 -
CHEMBL4475224 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4460257 IC50 = 50000.0 nM 4.30
CHEMBL4526803 IC50 = 60000.0 nM 4.22
CHEMBL4563763 IC50 = 60000.0 nM 4.22
CHEMBL13210 IC50 = 100000.0 nM 4.00
CHEMBL539219 IC50 = 100000.0 nM 4.00
CHEMBL4456172 IC50 = 500000.0 nM -
CHEMBL4561265 IC50 > 2000000.0 nM -
CHEMBL4468736 IC50 = 1300000.0 nM -
CHEMBL4554552 IC50 = 280000.0 nM -
CHEMBL4445639 IC50 = 500000.0 nM -
CHEMBL4475224 IC50 > 3000000.0 nM -