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Assay Detail

CHEMBL4424992

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant human FAAH using N-arachidonyl-7-amino-4-methylcoumarin as substrate preincubated for 20 mins followed by substrate addition and measured after 30 mins by fluorescence assay
13
Total Activities
7
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Fatty-acid amide hydrolase 1 (CHEMBL2243)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Plant-Based Modulators of Endocannabinoid Signaling.
Cunningham CW.
J Nat Prod (2019) 82 : 636 -646
PubMed 30816712 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 7 4.82 4.99
Efficacy 6 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2413160 2 4.99
CHEMBL2415106 2 4.97
CHEMBL2415105 2 4.96
CHEMBL2413162 2 4.86
CHEMBL1223510 2 4.78
CHEMBL2413161 2 4.38
CHEMBL2415104 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2413160 IC50 = 10300.0 nM 4.99
CHEMBL2415106 IC50 = 10800.0 nM 4.97
CHEMBL2415105 IC50 = 11000.0 nM 4.96
CHEMBL2413162 IC50 = 13700.0 nM 4.86
CHEMBL1223510 IC50 = 16700.0 nM 4.78
CHEMBL2413161 IC50 = 41800.0 nM 4.38
CHEMBL2415104 IC50 > 500000.0 nM -
CHEMBL1223510 Efficacy = 76.1 % -
CHEMBL2415105 Efficacy = 76.4 % -
CHEMBL2415106 Efficacy = 83.2 % -
CHEMBL2413160 Efficacy = 87.5 % -
CHEMBL2413161 Efficacy = 79.7 % -
CHEMBL2413162 Efficacy = 82.8 % -