Assay Detail
Binding
CHEMBL4425281
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Activation of human TRPA1 expressed in HEK293 cells assessed as increase in calcium level by fluo-4 dye based plate reader analysis
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Cell Type | HEK293 |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Transient receptor potential cation channel subfamily A member 1 (CHEMBL6007) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
Plumbagin, Juglone, and Boropinal as Novel TRPA1 Agonists.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| EC50 | 4 | 5.63 | 6.34 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL295316 | PLUMBAGIN | 1.0 | 1 | 6.34 |
| CHEMBL43612 | JUGLONE | — | 1 | 5.77 |
| CHEMBL233248 | ALLYL ISOTHIOCYANATE | 1.0 | 1 | 5.41 |
| CHEMBL2022666 | BOROPINAL | — | 1 | 5.01 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL295316 | PLUMBAGIN | EC50 | = | 460.0 | nM | 6.34 |
| CHEMBL43612 | JUGLONE | EC50 | = | 1700.0 | nM | 5.77 |
| CHEMBL233248 | ALLYL ISOTHIOCYANATE | EC50 | = | 3900.0 | nM | 5.41 |
| CHEMBL2022666 | BOROPINAL | EC50 | = | 9800.0 | nM | 5.01 |