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Assay Detail

CHEMBL4425307

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human His-tagged PDE3B catalytic domain expressed in Escherichia coli BL21(DE3) pLysS cells
2
Total Activities
2
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

cGMP-inhibited 3',5'-cyclic phosphodiesterase 3B (CHEMBL290)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

PDEStrIAn: A Phosphodiesterase Structure and Ligand Interaction Annotated Database As a Tool for Structure-Based Drug Design.
Jansen C, Kooistra AJ, Kanev GK, Leurs R, de Esch IJ, de Graaf C.
J Med Chem (2016) 59 : 7029 -7065
PubMed 26908025 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 2 8.10 9.57

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL131355 1 9.57
CHEMBL275084 ISOBUTYLMETHYLXANTHINE 1 6.62

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL131355 IC50 = 0.27 nM 9.57
CHEMBL275084 ISOBUTYLMETHYLXANTHINE IC50 = 242.0 nM 6.62