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Assay Detail

CHEMBL4425525

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of c-terminal GFP-tagged human ABCG2 expressed in MDCK2 cells preincubated for 30 mins before Hoechst 33342 addition by Hoechst 33342 accumulation assay
30
Total Activities
30
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Publication

Synthesis and Biological Evaluation of 4-Anilino-quinazolines and -quinolines as Inhibitors of Breast Cancer Resistance Protein (ABCG2).
Krapf MK, Wiese M.
J Med Chem (2016) 59 : 5449 -5461
PubMed 27148793 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 30 6.14 7.16

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4088022 1 7.16
CHEMBL4069582 1 7.10
CHEMBL2158554 1 6.82
CHEMBL1410991 1 6.69
CHEMBL488910 1 6.66
CHEMBL1307877 1 6.56
CHEMBL4558657 1 6.54
CHEMBL4085567 1 6.45
CHEMBL4528016 1 6.25
CHEMBL4458057 1 6.21
CHEMBL4453876 1 6.17
CHEMBL428402 1 6.15
CHEMBL4441055 1 6.10
CHEMBL118538 1 6.08
CHEMBL4530796 1 6.07
CHEMBL4446671 1 6.06
CHEMBL120099 1 6.03
CHEMBL4455303 1 6.02
CHEMBL4458072 1 5.97
CHEMBL4578761 1 5.97
CHEMBL4564993 1 5.97
CHEMBL1474597 1 5.94
CHEMBL4447490 1 5.92
CHEMBL4513952 1 5.84
CHEMBL1534709 1 5.83
CHEMBL4437626 1 5.79
CHEMBL4462578 1 5.72
CHEMBL4441537 1 5.67
CHEMBL4475288 1 5.25
CHEMBL1624741 1 5.20

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4088022 IC50 = 69.9 nM 7.16
CHEMBL4069582 IC50 = 80.0 nM 7.10
CHEMBL2158554 IC50 = 152.0 nM 6.82
CHEMBL1410991 IC50 = 204.0 nM 6.69
CHEMBL488910 IC50 = 221.0 nM 6.66
CHEMBL1307877 IC50 = 278.0 nM 6.56
CHEMBL4558657 IC50 = 289.0 nM 6.54
CHEMBL4085567 IC50 = 355.0 nM 6.45
CHEMBL4528016 IC50 = 556.0 nM 6.25
CHEMBL4458057 IC50 = 612.0 nM 6.21
CHEMBL4453876 IC50 = 672.0 nM 6.17
CHEMBL428402 IC50 = 704.0 nM 6.15
CHEMBL4441055 IC50 = 791.0 nM 6.10
CHEMBL118538 IC50 = 830.0 nM 6.08
CHEMBL4530796 IC50 = 846.0 nM 6.07
CHEMBL4446671 IC50 = 872.0 nM 6.06
CHEMBL120099 IC50 = 944.0 nM 6.03
CHEMBL4455303 IC50 = 951.0 nM 6.02
CHEMBL4458072 IC50 = 1070.0 nM 5.97
CHEMBL4578761 IC50 = 1060.0 nM 5.97
CHEMBL4564993 IC50 = 1060.0 nM 5.97
CHEMBL1474597 IC50 = 1150.0 nM 5.94
CHEMBL4447490 IC50 = 1190.0 nM 5.92
CHEMBL4513952 IC50 = 1460.0 nM 5.84
CHEMBL1534709 IC50 = 1480.0 nM 5.83
CHEMBL4437626 IC50 = 1630.0 nM 5.79
CHEMBL4462578 IC50 = 1900.0 nM 5.72
CHEMBL4441537 IC50 = 2130.0 nM 5.67
CHEMBL4475288 IC50 = 5650.0 nM 5.25
CHEMBL1624741 IC50 = 6380.0 nM 5.20