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Assay Detail

CHEMBL4425808

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human recombinant GST-tagged DDR1 (440 to 876 residues) expressed in baculovirus infected Sf9 insect cells using fluorescein-poly GAT as substrate preincubated for 1 hr measured after 1 hr in presence of ATP by TR-FRET LanthaScreen assay
11
Total Activities
11
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type Sf9
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Epithelial discoidin domain-containing receptor 1 (CHEMBL5319)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Structure-Based Design of Tetrahydroisoquinoline-7-carboxamides as Selective Discoidin Domain Receptor 1 (DDR1) Inhibitors.
Wang Z, Bian H, Bartual SG, Du W, Luo J, Zhao H, Zhang S, Mo C, Zhou Y, Xu Y, Tu Z, Ren X, Lu X, Brekken RA, Yao L, Bullock AN, Su J, Ding K.
J Med Chem (2016) 59 : 5911 -5916
PubMed 27219676 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 11 6.99 8.03

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4553037 1 8.03
CHEMBL4066114 1 7.61
CHEMBL4435100 1 7.44
CHEMBL4453539 1 6.51
CHEMBL4471343 1 6.49
CHEMBL4544384 1 6.48
CHEMBL4095191 1 6.36
CHEMBL4542873 1 -
CHEMBL4524615 1 -
CHEMBL4571618 1 -
CHEMBL4565209 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4553037 IC50 = 9.4 nM 8.03
CHEMBL4066114 IC50 = 24.3 nM 7.61
CHEMBL4435100 IC50 = 36.4 nM 7.44
CHEMBL4453539 IC50 = 309.0 nM 6.51
CHEMBL4471343 IC50 = 326.0 nM 6.49
CHEMBL4544384 IC50 = 328.0 nM 6.48
CHEMBL4095191 IC50 = 442.0 nM 6.36
CHEMBL4542873 IC50 > 3000.0 nM -
CHEMBL4524615 IC50 > 3000.0 nM -
CHEMBL4571618 IC50 > 1000.0 nM -
CHEMBL4565209 IC50 > 2000.0 nM -