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Assay Detail

CHEMBL4428225

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of thioredoxin reductase in human A549 cells by DTNB dye based microplate spectrophotometry
7
Total Activities
7
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type A549
Confidence 5 — Multiple protein complex / RNA
Curated By Autocuration

Target

Thioredoxin reductase (CHEMBL2096978)
Type PROTEIN FAMILY
Organism Homo sapiens

Publication

Discovery of New Monocarbonyl Ligustrazine-Curcumin Hybrids for Intervention of Drug-Sensitive and Drug-Resistant Lung Cancer.
Ai Y, Zhu B, Ren C, Kang F, Li J, Huang Z, Lai Y, Peng S, Ding K, Tian J, Zhang Y.
J Med Chem (2016) 59 : 1747 -1760
PubMed 26891099 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 7 5.32 5.83

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4451948 1 5.83
CHEMBL4475597 1 5.63
CHEMBL4458105 1 5.38
CHEMBL4442682 1 5.38
CHEMBL4521088 1 5.33
CHEMBL140 CURCUMIN 3.0 1 4.39
CHEMBL303697 LIGUSTRAZINE 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4451948 IC50 = 1490.0 nM 5.83
CHEMBL4475597 IC50 = 2360.0 nM 5.63
CHEMBL4458105 IC50 = 4170.0 nM 5.38
CHEMBL4442682 IC50 = 4120.0 nM 5.38
CHEMBL4521088 IC50 = 4730.0 nM 5.33
CHEMBL140 CURCUMIN IC50 = 40560.0 nM 4.39
CHEMBL303697 LIGUSTRAZINE IC50 > 200000.0 nM -