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Assay Detail

CHEMBL4429374

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of BTK in human RamosB cells assessed as suppression of BCR/anti-IgM stimulated calcium flux pre-incubated for 1 hr followed by BCR/anti-IgM stimulation
13
Total Activities
13
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Tyrosine-protein kinase BTK (CHEMBL5251)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Small Molecule Reversible Inhibitors of Bruton's Tyrosine Kinase (BTK): Structure-Activity Relationships Leading to the Identification of 7-(2-Hydroxypropan-2-yl)-4-[2-methyl-3-(4-oxo-3,4-dihydroquinazolin-3-yl)phenyl]-9H-carbazole-1-carboxamide (BMS-935177).
De Lucca GV, Shi Q, Liu Q, Batt DG, Beaudoin Bertrand M, Rampulla R, Mathur A, Discenza L, D'Arienzo C, Dai J, Obermeier M, Vickery R, Zhang Y, Yang Z, Marathe P, Tebben AJ, Muckelbauer JK, Chang CJ, Zhang H, Gillooly K, Taylor T, Pattoli MA, Skala S, Kukral DW, McIntyre KW, Salter-Cid L, Fura A, Burke JR, Barrish JC, Carter PH, Tino JA.
J Med Chem (2016) 59 : 7915 -7935
PubMed 27531604 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 13 7.27 7.75

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4473176 1 7.75
CHEMBL4550390 1 7.66
CHEMBL4467132 1 7.62
CHEMBL4465380 1 7.58
CHEMBL3918580 1 7.58
CHEMBL3977725 1 7.52
CHEMBL4460221 1 7.47
CHEMBL4513599 1 7.40
CHEMBL4543505 1 7.32
CHEMBL4554269 1 6.84
CHEMBL4439761 1 6.81
CHEMBL4567605 1 6.62
CHEMBL3939772 1 6.39

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4473176 IC50 = 18.0 nM 7.75
CHEMBL4550390 IC50 = 22.0 nM 7.66
CHEMBL4467132 IC50 = 24.0 nM 7.62
CHEMBL4465380 IC50 = 26.0 nM 7.58
CHEMBL3918580 IC50 = 26.0 nM 7.58
CHEMBL3977725 IC50 = 30.0 nM 7.52
CHEMBL4460221 IC50 = 34.0 nM 7.47
CHEMBL4513599 IC50 = 40.0 nM 7.40
CHEMBL4543505 IC50 = 48.0 nM 7.32
CHEMBL4554269 IC50 = 144.0 nM 6.84
CHEMBL4439761 IC50 = 154.0 nM 6.81
CHEMBL4567605 IC50 = 238.0 nM 6.62
CHEMBL3939772 IC50 = 410.0 nM 6.39