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Assay Detail

CHEMBL4430043

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human PI3Kalpha expressed in rat-1 fibroblasts assessed as reduction in Akt phosphorylation at Ser 473 after 1 hr by alphascreen assay
12
Total Activities
12
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Publication

Discovery and Pharmacological Characterization of Novel Quinazoline-Based PI3K Delta-Selective Inhibitors.
Hoegenauer K, Soldermann N, Stauffer F, Furet P, Graveleau N, Smith AB, Hebach C, Hollingworth GJ, Lewis I, Gutmann S, Rummel G, Knapp M, Wolf RM, Blanz J, Feifel R, Burkhart C, Zécri F.
ACS Med Chem Lett (2016) 7 : 762 -767
PubMed 27563400 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 12 5.84 6.40

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4453497 1 6.40
CHEMBL4521888 1 6.16
CHEMBL4588314 1 5.96
CHEMBL4437791 1 5.94
CHEMBL3944013 1 5.88
CHEMBL4467868 1 5.81
CHEMBL4581278 1 5.81
CHEMBL4454454 1 5.74
CHEMBL4469006 1 5.68
CHEMBL3947814 1 5.67
CHEMBL4460621 1 5.60
CHEMBL3977066 1 5.46

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4453497 IC50 = 400.0 nM 6.40
CHEMBL4521888 IC50 = 690.0 nM 6.16
CHEMBL4588314 IC50 = 1090.0 nM 5.96
CHEMBL4437791 IC50 = 1140.0 nM 5.94
CHEMBL3944013 IC50 = 1320.0 nM 5.88
CHEMBL4467868 IC50 = 1560.0 nM 5.81
CHEMBL4581278 IC50 = 1560.0 nM 5.81
CHEMBL4454454 IC50 = 1820.0 nM 5.74
CHEMBL4469006 IC50 = 2100.0 nM 5.68
CHEMBL3947814 IC50 = 2120.0 nM 5.67
CHEMBL4460621 IC50 = 2490.0 nM 5.60
CHEMBL3977066 IC50 = 3440.0 nM 5.46