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Assay Detail

CHEMBL4432181

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of MB-COMT in Wistar rat brain assessed as metanephrine formation preincubated for 20 mins followed by addition of adrenaline as substrate and SAM measured after 15 mins by chromatographic analysis
22
Total Activities
16
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Tissue Brain
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Catechol O-methyltransferase (CHEMBL2372)
Type SINGLE PROTEIN
Organism Rattus norvegicus

Publication

Development of Blood-Brain Barrier Permeable Nitrocatechol-Based Catechol O-Methyltransferase Inhibitors with Reduced Potential for Hepatotoxicity.
Silva T, Mohamed T, Shakeri A, Rao PP, Martínez-González L, Pérez DI, Martínez A, Valente MJ, Garrido J, Uriarte E, Serrão P, Soares-da-Silva P, Remião F, Borges F.
J Med Chem (2016) 59 : 7584 -7597
PubMed 27463695 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 22 8.31 9.08

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4463709 1 9.08
CHEMBL1324 TOLCAPONE 4.0 2 9.04
CHEMBL4437043 1 8.79
CHEMBL4447349 2 8.73
CHEMBL953 ENTACAPONE 4.0 2 8.52
CHEMBL4448310 1 8.50
CHEMBL4543735 2 8.43
CHEMBL4444830 2 8.32
CHEMBL481644 2 8.31
CHEMBL4437795 1 8.26
CHEMBL137555 1 8.24
CHEMBL145 CAFFEIC ACID 3.0 1 7.03
CHEMBL4521833 1 7.00
CHEMBL4472878 1 6.74
CHEMBL133714 BENZYL CAFFEATE 1 -
CHEMBL319244 CAFFEIC ACID PHENETHYL ESTER 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4463709 IC50 = 0.84 nM 9.08
CHEMBL1324 TOLCAPONE IC50 = 0.91 nM 9.04
CHEMBL1324 TOLCAPONE IC50 = 0.912 nM 9.04
CHEMBL4437043 IC50 = 1.64 nM 8.79
CHEMBL4447349 IC50 = 1.862 nM 8.73
CHEMBL4447349 IC50 = 1.92 nM 8.72
CHEMBL953 ENTACAPONE IC50 = 3.02 nM 8.52
CHEMBL4448310 IC50 = 3.14 nM 8.50
CHEMBL953 ENTACAPONE IC50 = 3.47 nM 8.46
CHEMBL4543735 IC50 = 3.715 nM 8.43
CHEMBL4543735 IC50 = 3.77 nM 8.42
CHEMBL4444830 IC50 = 4.78 nM 8.32
CHEMBL4444830 IC50 = 4.786 nM 8.32
CHEMBL481644 IC50 = 4.898 nM 8.31
CHEMBL481644 IC50 = 5.02 nM 8.30
CHEMBL4437795 IC50 = 5.56 nM 8.26
CHEMBL137555 IC50 = 5.79 nM 8.24
CHEMBL145 CAFFEIC ACID IC50 = 92.82 nM 7.03
CHEMBL4521833 IC50 = 100.71 nM 7.00
CHEMBL4472878 IC50 = 180.39 nM 6.74
CHEMBL133714 BENZYL CAFFEATE IC50 - -
CHEMBL319244 CAFFEIC ACID PHENETHYL ESTER IC50 - -