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Assay Detail

CHEMBL4432503

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant Helicobacter pylori urease expressed in Escherichia coli rosetta DE3 after 120 mins by Berthelot colorimetric analysis
6
Total Activities
6
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Helicobacter pylori
Confidence 6 — Multiple protein complex / RNA
Curated By Autocuration

Target

Urease subunit alpha/Urease subunit beta (CHEMBL3885651)
Type PROTEIN COMPLEX
Organism Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacterpylori)

Publication

1,2-Benzisoselenazol-3(2H)-one Derivatives As a New Class of Bacterial Urease Inhibitors.
Macegoniuk K, Grela E, Palus J, Rudzińska-Szostak E, Grabowiecka A, Biernat M, Berlicki Ł.
J Med Chem (2016) 59 : 8125 -8133
PubMed 27524377 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 6 5.86 6.74

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4575318 1 6.74
CHEMBL51085 EBSELEN 3.0 1 6.65
CHEMBL385817 EBSELEN DISELENIDE 1 6.19
CHEMBL4471428 1 6.04
CHEMBL4539240 1 5.49
CHEMBL734 ACETOHYDROXAMIC ACID 4.0 1 4.02

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4575318 Ki = 184.0 nM 6.74
CHEMBL51085 EBSELEN Ki = 226.0 nM 6.65
CHEMBL385817 EBSELEN DISELENIDE Ki = 651.0 nM 6.19
CHEMBL4471428 Ki = 915.0 nM 6.04
CHEMBL4539240 Ki = 3259.0 nM 5.49
CHEMBL734 ACETOHYDROXAMIC ACID Ki = 96000.0 nM 4.02