Assay Detail
Binding
CHEMBL4432927
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Binding affinity to recombinant hexa-histidine-tagged human full-length N-GLY1 catalytic domain expressed in Escherichia coli BL21 (DE3) by thermal shift assay
9
Total Activities
9
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase (CHEMBL4523429) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
Novel small molecule binders of human N-glycanase 1, a key player in the endoplasmic reticulum associated degradation pathway.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| Kd | 9 | 4.19 | 4.48 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL1549362 | — | — | 1 | 4.48 |
| CHEMBL1554 | DACTINOMYCIN | 4.0 | 1 | 4.05 |
| CHEMBL311226 | CASTANOSPERMINE | -1.0 | 1 | 4.03 |
| CHEMBL4468390 | — | — | 1 | - |
| CHEMBL4297023 | — | — | 1 | - |
| CHEMBL1866245 | — | — | 1 | - |
| CHEMBL1482516 | BATILOL | 2.0 | 1 | - |
| CHEMBL2144547 | — | — | 1 | - |
| CHEMBL1451886 | — | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL1549362 | — | Kd | = | 32900.0 | nM | 4.48 |
| CHEMBL1554 | DACTINOMYCIN | Kd | = | 88500.0 | nM | 4.05 |
| CHEMBL311226 | CASTANOSPERMINE | Kd | = | 94300.0 | nM | 4.03 |
| CHEMBL4468390 | — | Kd | = | 127700.0 | nM | - |
| CHEMBL4297023 | — | Kd | = | 150900.0 | nM | - |
| CHEMBL1866245 | — | Kd | = | 162300.0 | nM | - |
| CHEMBL1482516 | BATILOL | Kd | = | 221500.0 | nM | - |
| CHEMBL2144547 | — | Kd | = | 275300.0 | nM | - |
| CHEMBL1451886 | — | Kd | = | 315300.0 | nM | - |