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Assay Detail

CHEMBL4433277

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [3H]-N-alpha-methylhistamine from human recombinant full length H3R expressed in HEK293 cell membranes incubated for 90 mins by liquid scintillation counting method
14
Total Activities
14
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Histamine H3 receptor (CHEMBL264)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Biphenyloxy-alkyl-piperidine and azepane derivatives as histamine H<sub>3</sub> receptor ligands.
Łażewska D, Kaleta M, Schwed JS, Karcz T, Mogilski S, Latacz G, Olejarz A, Siwek A, Kubacka M, Lubelska A, Honkisz E, Handzlik J, Filipek B, Stark H, Kieć-Kononowicz K.
Bioorg Med Chem (2017) 25 : 5341 -5354
PubMed 28797771 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 14 7.12 7.93

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL462605 PITOLISANT 4.0 1 7.93
CHEMBL4445028 1 7.75
CHEMBL4540849 1 7.60
CHEMBL4586133 1 7.47
CHEMBL4585724 1 7.12
CHEMBL4450520 1 7.05
CHEMBL4469232 1 7.04
CHEMBL4437238 1 6.94
CHEMBL4514837 1 6.91
CHEMBL4159018 1 6.88
CHEMBL4552629 1 6.82
CHEMBL4465338 1 6.80
CHEMBL4553559 1 6.80
CHEMBL4467138 1 6.58

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL462605 PITOLISANT Ki = 11.7 nM 7.93
CHEMBL4445028 Ki = 18.0 nM 7.75
CHEMBL4540849 Ki = 25.0 nM 7.60
CHEMBL4586133 Ki = 34.0 nM 7.47
CHEMBL4585724 Ki = 76.0 nM 7.12
CHEMBL4450520 Ki = 89.0 nM 7.05
CHEMBL4469232 Ki = 92.0 nM 7.04
CHEMBL4437238 Ki = 115.0 nM 6.94
CHEMBL4514837 Ki = 122.0 nM 6.91
CHEMBL4159018 Ki = 131.0 nM 6.88
CHEMBL4552629 Ki = 151.0 nM 6.82
CHEMBL4465338 Ki = 159.0 nM 6.80
CHEMBL4553559 Ki = 157.0 nM 6.80
CHEMBL4467138 Ki = 266.0 nM 6.58