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Assay Detail

CHEMBL4433438

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human H3R expressed in methylhistamine-induced human H3-bla U2OS cells incubated for 30 mins by beta-lactamase complementation technology
18
Total Activities
18
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type U2OS
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Histamine H3 receptor (CHEMBL264)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Design, synthesis, and evaluation of isoflavone analogs as multifunctional agents for the treatment of Alzheimer's disease.
Wang D, Hu M, Li X, Zhang D, Chen C, Fu J, Shao S, Shi G, Zhou Y, Wu S, Zhang T.
Eur J Med Chem (2019) 168 : 207 -220
PubMed 30822710 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 18 6.14 6.57

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4568293 1 6.57
CHEMBL4552051 1 6.39
CHEMBL4554976 1 6.37
CHEMBL4516496 1 6.28
CHEMBL4439461 1 6.14
CHEMBL260374 THIOPERAMIDE 1 5.99
CHEMBL4554072 1 5.71
CHEMBL4586884 1 5.68
CHEMBL4471251 1 -
CHEMBL4476554 1 -
CHEMBL4450260 1 -
CHEMBL4445115 1 -
CHEMBL4583406 1 -
CHEMBL4438929 1 -
CHEMBL4555824 1 -
CHEMBL4543223 1 -
CHEMBL502 DONEPEZIL 4.0 1 -
CHEMBL636 RIVASTIGMINE 4.0 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4568293 IC50 = 270.0 nM 6.57
CHEMBL4552051 IC50 = 410.0 nM 6.39
CHEMBL4554976 IC50 = 430.0 nM 6.37
CHEMBL4516496 IC50 = 520.0 nM 6.28
CHEMBL4439461 IC50 = 730.0 nM 6.14
CHEMBL260374 THIOPERAMIDE IC50 = 1030.0 nM 5.99
CHEMBL4554072 IC50 = 1940.0 nM 5.71
CHEMBL4586884 IC50 = 2070.0 nM 5.68
CHEMBL4471251 IC50 > 50000.0 nM -
CHEMBL4476554 IC50 - -
CHEMBL4450260 IC50 > 50000.0 nM -
CHEMBL4445115 IC50 - -
CHEMBL4583406 IC50 - -
CHEMBL4438929 IC50 > 10000.0 nM -
CHEMBL4555824 IC50 > 50000.0 nM -
CHEMBL4543223 IC50 > 50000.0 nM -
CHEMBL502 DONEPEZIL IC50 - -
CHEMBL636 RIVASTIGMINE IC50 - -