Assay Detail
Binding
CHEMBL4434086
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of human 6His-TEV-TNIK(1 to 325) expressed in baculovirus-infected sf21 cells using LRRKtide as substrate preincubated for 30 mins followed by substrate addition and measured after 75 to 90 mins by ADP-Glo Reagent based method
9
Total Activities
8
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Cell Type | Sf21 |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
TRAF2 and NCK-interacting protein kinase (CHEMBL4527) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
Tool inhibitors and assays to interrogate the biology of the TRAF2 and NCK interacting kinase.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 9 | 6.48 | 8.00 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL3754283 | — | — | 1 | 8.00 |
| CHEMBL4471729 | — | — | 1 | 7.90 |
| CHEMBL4558962 | — | — | 1 | 7.10 |
| CHEMBL4527085 | — | — | 1 | 6.90 |
| CHEMBL3262590 | — | — | 2 | 6.82 |
| CHEMBL4451091 | — | — | 1 | 5.40 |
| CHEMBL4558988 | — | — | 1 | 4.80 |
| CHEMBL4465524 | — | — | 1 | 4.60 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL3754283 | — | IC50 | = | 10.0 | nM | 8.00 |
| CHEMBL4471729 | — | IC50 | = | 12.59 | nM | 7.90 |
| CHEMBL4558962 | — | IC50 | = | 79.43 | nM | 7.10 |
| CHEMBL4527085 | — | IC50 | = | 125.89 | nM | 6.90 |
| CHEMBL3262590 | — | IC50 | = | 150.0 | nM | 6.82 |
| CHEMBL3262590 | — | IC50 | = | 158.49 | nM | 6.80 |
| CHEMBL4451091 | — | IC50 | = | 3981.07 | nM | 5.40 |
| CHEMBL4558988 | — | IC50 | = | 15848.93 | nM | 4.80 |
| CHEMBL4465524 | — | IC50 | = | 25118.86 | nM | 4.60 |