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Assay Detail

CHEMBL4434583

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human PDL1 expressed in CHO-K1 cells co-expressing aAPC assessed as reduction in PDL1 interaction with human PD1 expressed in Jurkat T cells incubated for 18 hrs by Bio-Glo reagent based luminescence assay
7
Total Activities
7
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 7 — Homologous single protein target
Curated By Autocuration

Publication

Symmetry-based ligand design and evaluation of small molecule inhibitors of programmed cell death-1/programmed death-ligand 1 interaction.
Kawashita S, Aoyagi K, Yamanaka H, Hantani R, Naruoka S, Tanimoto A, Hori Y, Toyonaga Y, Fukushima K, Miyazaki S, Hantani Y.
Bioorg Med Chem Lett (2019) 29 : 2464 -2467
PubMed 31351692 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 7 5.37 6.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4473436 1 6.00
CHEMBL4456990 1 5.08
CHEMBL4578475 1 5.02
CHEMBL4473243 1 -
CHEMBL4515820 1 -
CHEMBL4472332 1 -
CHEMBL4460716 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4473436 EC50 = 1000.0 nM 6.00
CHEMBL4456990 EC50 = 8400.0 nM 5.08
CHEMBL4578475 EC50 = 9500.0 nM 5.02
CHEMBL4473243 EC50 > 100000.0 nM -
CHEMBL4515820 EC50 - -
CHEMBL4472332 EC50 > 30000.0 nM -
CHEMBL4460716 EC50 - -