Assay Detail
Binding
CHEMBL4480181
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of recombinant full length human N-terminal GST-tagged C-terminal His6-tagged MEK1 expressed in Escherichia coli using myelin basic protein as substrate in presence of activated BRAF and ERK2 by ELISA
13
Total Activities
13
Compounds Tested
2
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Dual specificity mitogen-activated protein kinase kinase 1 (CHEMBL3587) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
Discovery of N-(benzyloxy)-1,3-diphenyl-1H-pyrazole-4-carboxamide derivatives as potential antiproliferative agents by inhibiting MEK.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| Inhibition | 11 | - | - |
| IC50 | 2 | 7.05 | 7.05 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL34704 | — | — | 1 | 7.05 |
| CHEMBL4575118 | — | — | 1 | 7.04 |
| CHEMBL4545932 | — | — | 1 | - |
| CHEMBL4586091 | — | — | 1 | - |
| CHEMBL4528906 | — | — | 1 | - |
| CHEMBL4556810 | — | — | 1 | - |
| CHEMBL4560047 | — | — | 1 | - |
| CHEMBL4580386 | — | — | 1 | - |
| CHEMBL4577383 | — | — | 1 | - |
| CHEMBL4575979 | — | — | 1 | - |
| CHEMBL4580566 | — | — | 1 | - |
| CHEMBL4568694 | — | — | 1 | - |
| CHEMBL4575359 | — | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL34704 | — | IC50 | = | 89.0 | nM | 7.05 |
| CHEMBL4575118 | — | IC50 | = | 91.0 | nM | 7.04 |
| CHEMBL4545932 | — | Inhibition | - | % | - | |
| CHEMBL4586091 | — | Inhibition | - | % | - | |
| CHEMBL4528906 | — | Inhibition | - | % | - | |
| CHEMBL4556810 | — | Inhibition | - | % | - | |
| CHEMBL4560047 | — | Inhibition | - | % | - | |
| CHEMBL4580386 | — | Inhibition | - | % | - | |
| CHEMBL4577383 | — | Inhibition | - | % | - | |
| CHEMBL4575979 | — | Inhibition | - | % | - | |
| CHEMBL4580566 | — | Inhibition | - | % | - | |
| CHEMBL4568694 | — | Inhibition | - | % | - | |
| CHEMBL4575359 | — | Inhibition | - | % | - |