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Assay Detail

CHEMBL4480596

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Antagonist activity at histamine H3 receptor in guinea pig jejunum assessed as effect on pyrilamine-induced inhibition of electrically evoked contraction treated 20 mins after pyrilamine stimulation
9
Total Activities
9
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Cavia porcellus
Tissue Jejunum
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Histamine H3 receptor (CHEMBL5076)
Type SINGLE PROTEIN
Organism Cavia porcellus

Publication

Design, synthesis, and <i>in vitro</i> and <i>in vivo</i> characterization of 1-{4-[4-(substituted)piperazin-1-yl]butyl}guanidines and their piperidine analogues as histamine H<sub>3</sub> receptor antagonists.
Staszewski M, Stasiak A, Karcz T, McNaught Flores D, Fogel WA, Kieć-Kononowicz K, Leurs R, Walczyński K.
Medchemcomm (2019) 10 : 234 -251
PubMed 30881612 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Kd 9 7.57 8.98

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL260374 THIOPERAMIDE 1 8.98
CHEMBL4585110 1 8.35
CHEMBL4482871 1 8.10
CHEMBL4514561 1 7.99
CHEMBL4579190 1 7.97
CHEMBL4546027 1 7.90
CHEMBL4517581 1 7.30
CHEMBL4550675 1 5.78
CHEMBL4591503 1 5.78

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL260374 THIOPERAMIDE Kd = 1.047 nM 8.98
CHEMBL4585110 Kd = 4.467 nM 8.35
CHEMBL4482871 Kd = 7.943 nM 8.10
CHEMBL4514561 Kd = 10.23 nM 7.99
CHEMBL4579190 Kd = 10.72 nM 7.97
CHEMBL4546027 Kd = 12.59 nM 7.90
CHEMBL4517581 Kd = 50.12 nM 7.30
CHEMBL4550675 Kd = 1659.59 nM 5.78
CHEMBL4591503 Kd = 1659.59 nM 5.78